Specific tissues, such as cartilage, undergo
mechanical solicitation under their normal performance in
human body. In this sense, it seems necessary that proper
tissue engineering strategies of these tissues should incorporate
mechanical solicitations during cell culture, in order
to properly evaluate the influence of the mechanical stimulus.
This work reports on a user-friendly bioreactor suitable
for applying controlled mechanical stimulation—amplitude
and frequency—to three-dimensional scaffolds. Its design
and main components are described, as well as its operation
characteristics. The modular design allows easy cleaning
and operating under laminar hood. Different protocols for
the sterilization of the hermetic enclosure are tested and
ensure lack of observable contaminations, complying with
the requirements to be used for cell culture. The cell viability
study was performed with KUM5 cells.