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Design and validation of a biomechanical bioreactor for cartilage tissue culture

Journal Article


Abstract


  • Specific tissues, such as cartilage, undergo

    mechanical solicitation under their normal performance in

    human body. In this sense, it seems necessary that proper

    tissue engineering strategies of these tissues should incorporate

    mechanical solicitations during cell culture, in order

    to properly evaluate the influence of the mechanical stimulus.

    This work reports on a user-friendly bioreactor suitable

    for applying controlled mechanical stimulation—amplitude

    and frequency—to three-dimensional scaffolds. Its design

    and main components are described, as well as its operation

    characteristics. The modular design allows easy cleaning

    and operating under laminar hood. Different protocols for

    the sterilization of the hermetic enclosure are tested and

    ensure lack of observable contaminations, complying with

    the requirements to be used for cell culture. The cell viability

    study was performed with KUM5 cells.

UOW Authors


  •   Correia, Vítor (external author)
  •   Panadero, J A. (external author)
  •   Ribeiro, Clarisse (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Rocha, José Gerardo (external author)
  •   Gomez Ribelles, J L. (external author)
  •   Lanceros-Méndez, Senentxu (external author)

Publication Date


  • 2016

Citation


  • Correia, V., Panadero, J. A., Ribeiro, C., Sencadas, V., Rocha, J. G., Gomez Ribelles, J. L. & Lanceros-Mendez, S. (2016). Design and validation of a biomechanical bioreactor for cartilage tissue culture. Biomechanics and Modeling in Mechanobiology, 15 (2), 471-478.

Scopus Eid


  • 2-s2.0-84961148003

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 471

End Page


  • 478

Volume


  • 15

Issue


  • 2

Place Of Publication


  • Germany

Abstract


  • Specific tissues, such as cartilage, undergo

    mechanical solicitation under their normal performance in

    human body. In this sense, it seems necessary that proper

    tissue engineering strategies of these tissues should incorporate

    mechanical solicitations during cell culture, in order

    to properly evaluate the influence of the mechanical stimulus.

    This work reports on a user-friendly bioreactor suitable

    for applying controlled mechanical stimulation—amplitude

    and frequency—to three-dimensional scaffolds. Its design

    and main components are described, as well as its operation

    characteristics. The modular design allows easy cleaning

    and operating under laminar hood. Different protocols for

    the sterilization of the hermetic enclosure are tested and

    ensure lack of observable contaminations, complying with

    the requirements to be used for cell culture. The cell viability

    study was performed with KUM5 cells.

UOW Authors


  •   Correia, Vítor (external author)
  •   Panadero, J A. (external author)
  •   Ribeiro, Clarisse (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Rocha, José Gerardo (external author)
  •   Gomez Ribelles, J L. (external author)
  •   Lanceros-Méndez, Senentxu (external author)

Publication Date


  • 2016

Citation


  • Correia, V., Panadero, J. A., Ribeiro, C., Sencadas, V., Rocha, J. G., Gomez Ribelles, J. L. & Lanceros-Mendez, S. (2016). Design and validation of a biomechanical bioreactor for cartilage tissue culture. Biomechanics and Modeling in Mechanobiology, 15 (2), 471-478.

Scopus Eid


  • 2-s2.0-84961148003

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 471

End Page


  • 478

Volume


  • 15

Issue


  • 2

Place Of Publication


  • Germany