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Antibacterial and Antifungal Activity of Poly(Lactic Acid)-Bovine Lactoferrin Nanofiber Membranes

Journal Article


Abstract


  • Antimicrobial materials have become relevant for local therapies preventing microbial resistance induced by systemic antibiotic treatments. This work reports the development of electrospun poly(lactic acid) (PLLA) nanofiber membranes loaded with bovine lactoferrin (bLF) up to 20 wt%. The membranes present smooth and nondefective fibers with mean diameters between 717 ± 197 and 495 ± 127 nm, and an overall porosity of ≈80%. The hydrophobicity of the PLLA membranes is reduced by the presence of bLF. The release profile of bLF correlates with an anomalous transport model, with 17.7 ± 3.6% being released over 7 weeks. The nanofiber mats show no cytotoxicity on human skin fibroblasts and even promote cell proliferation after short exposure periods. Furthermore, the developed membranes display antifungal activity against Aspergillus nidulans by inhibiting spore germination and mycelial growth. These results evidence the strong potential of bLF-PLLA nanofiber membranes to be used as antifungal dressings.

UOW Authors


  •   Machado, Raul (external author)
  •   Da Costa, André (external author)
  •   Silva, D M. (external author)
  •   Gomes, A C. (external author)
  •   Casal, Margarida (external author)
  •   Gomes da Silva Sencadas, Vitor

Publication Date


  • 2018

Citation


  • Machado, R., da Costa, A., Silva, D. M., Gomes, A. C., Casal, M. & Sencadas, V. (2018). Antibacterial and Antifungal Activity of Poly(Lactic Acid)-Bovine Lactoferrin Nanofiber Membranes. Macromolecular Bioscience, 18 (3), 1700324-1-1700324-10.

Scopus Eid


  • 2-s2.0-85040675171

Start Page


  • 1700324-1

End Page


  • 1700324-10

Volume


  • 18

Issue


  • 3

Place Of Publication


  • Germany

Abstract


  • Antimicrobial materials have become relevant for local therapies preventing microbial resistance induced by systemic antibiotic treatments. This work reports the development of electrospun poly(lactic acid) (PLLA) nanofiber membranes loaded with bovine lactoferrin (bLF) up to 20 wt%. The membranes present smooth and nondefective fibers with mean diameters between 717 ± 197 and 495 ± 127 nm, and an overall porosity of ≈80%. The hydrophobicity of the PLLA membranes is reduced by the presence of bLF. The release profile of bLF correlates with an anomalous transport model, with 17.7 ± 3.6% being released over 7 weeks. The nanofiber mats show no cytotoxicity on human skin fibroblasts and even promote cell proliferation after short exposure periods. Furthermore, the developed membranes display antifungal activity against Aspergillus nidulans by inhibiting spore germination and mycelial growth. These results evidence the strong potential of bLF-PLLA nanofiber membranes to be used as antifungal dressings.

UOW Authors


  •   Machado, Raul (external author)
  •   Da Costa, André (external author)
  •   Silva, D M. (external author)
  •   Gomes, A C. (external author)
  •   Casal, Margarida (external author)
  •   Gomes da Silva Sencadas, Vitor

Publication Date


  • 2018

Citation


  • Machado, R., da Costa, A., Silva, D. M., Gomes, A. C., Casal, M. & Sencadas, V. (2018). Antibacterial and Antifungal Activity of Poly(Lactic Acid)-Bovine Lactoferrin Nanofiber Membranes. Macromolecular Bioscience, 18 (3), 1700324-1-1700324-10.

Scopus Eid


  • 2-s2.0-85040675171

Start Page


  • 1700324-1

End Page


  • 1700324-10

Volume


  • 18

Issue


  • 3

Place Of Publication


  • Germany