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Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells

Journal Article


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Abstract


  • The dorsal root ganglia (DRG) consist of a multitude of sensory neuronal subtypes that function to relay sensory stimuli, including temperature, pressure, pain and position to the central nervous system. Our knowledge of DRG sensory neurons have been predominantly driven by animal studies and considerably less is known about the human DRG. Human embryonic stem cells (hESC) are valuable resource to help close this gap. Our previous studies reported an efficient system for deriving neural crest and DRG sensory neurons from hESC. Here we show that this differentiation system gives rise to heterogeneous populations of sensory neuronal subtypes as demonstrated by phenotypic and functional analyses. Furthermore, using microelectrode arrays the maturation rate of the hESC-derived sensory neuronal cultures was monitored over 8 weeks in culture, showing their spontaneous firing activities starting at about 12 days post-differentiation and reaching maximum firing at about 6 weeks. These studies are highly valuable for developing an in vitro platform to study the diversity of sensory neuronal subtypes found within the human DRG.

Authors


  •   Alshawaf, Abdullah (external author)
  •   Viventi, Serena (external author)
  •   Qiu, Wanzhi (external author)
  •   D'Abaco, Giovanna (external author)
  •   Nayagam, Bryony A. (external author)
  •   Erlichster, Michael (external author)
  •   Chana, Gursharan (external author)
  •   Everall, Ian (external author)
  •   Ivanusic, Jason (external author)
  •   Skafidas, Efstratios (external author)
  •   Dottori, Mirella

Publication Date


  • 2018

Citation


  • Alshawaf, A., Viventi, S., Qiu, W., D'Abaco, G., Nayagam, B., Erlichster, M., Chana, G., Everall, I., Ivanusic, J., Skafidas, E. & Dottori, M. (2018). Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells. Scientific Reports, 8 603-1-603-12.

Scopus Eid


  • 2-s2.0-85040507127

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2232&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1205

Start Page


  • 603-1

End Page


  • 603-12

Volume


  • 8

Place Of Publication


  • United Kingdom

Abstract


  • The dorsal root ganglia (DRG) consist of a multitude of sensory neuronal subtypes that function to relay sensory stimuli, including temperature, pressure, pain and position to the central nervous system. Our knowledge of DRG sensory neurons have been predominantly driven by animal studies and considerably less is known about the human DRG. Human embryonic stem cells (hESC) are valuable resource to help close this gap. Our previous studies reported an efficient system for deriving neural crest and DRG sensory neurons from hESC. Here we show that this differentiation system gives rise to heterogeneous populations of sensory neuronal subtypes as demonstrated by phenotypic and functional analyses. Furthermore, using microelectrode arrays the maturation rate of the hESC-derived sensory neuronal cultures was monitored over 8 weeks in culture, showing their spontaneous firing activities starting at about 12 days post-differentiation and reaching maximum firing at about 6 weeks. These studies are highly valuable for developing an in vitro platform to study the diversity of sensory neuronal subtypes found within the human DRG.

Authors


  •   Alshawaf, Abdullah (external author)
  •   Viventi, Serena (external author)
  •   Qiu, Wanzhi (external author)
  •   D'Abaco, Giovanna (external author)
  •   Nayagam, Bryony A. (external author)
  •   Erlichster, Michael (external author)
  •   Chana, Gursharan (external author)
  •   Everall, Ian (external author)
  •   Ivanusic, Jason (external author)
  •   Skafidas, Efstratios (external author)
  •   Dottori, Mirella

Publication Date


  • 2018

Citation


  • Alshawaf, A., Viventi, S., Qiu, W., D'Abaco, G., Nayagam, B., Erlichster, M., Chana, G., Everall, I., Ivanusic, J., Skafidas, E. & Dottori, M. (2018). Phenotypic and Functional Characterization of Peripheral Sensory Neurons derived from Human Embryonic Stem Cells. Scientific Reports, 8 603-1-603-12.

Scopus Eid


  • 2-s2.0-85040507127

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2232&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1205

Start Page


  • 603-1

End Page


  • 603-12

Volume


  • 8

Place Of Publication


  • United Kingdom