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Increased accuracy of starch granule type quantification using mixture distributions

Journal Article


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Abstract


  • Background: The proportion of granule types in wheat starch is an important characteristic that can affect its functionality. It is widely accepted that granule types are either large, disc-shaped A-type granules or small, spherical B-type granules. Additionally, there are some reports of the tiny C-type granules. The differences between these granule types are due to its carbohydrate composition and crystallinity which is highly, but not perfectly, correlated with the granule size. A majority of the studies that have considered granule types analyse them based on a size threshold rather than chemical composition. This is understandable due to the expense of separating starch into different types. While the use of a size threshold to classify granule type is a low-cost measure, this results in misclassification. We present an alternative, statistical method to quantify the proportion of granule types by a fit of the mixture distribution, along with an R package, a web based app and a video tutorial for how to use the web app to enable its straightforward application. Results: Our results show that the reliability of the gen otypic effects increase approximately 60% using the proportions of the A-type and B-type granule estimated by the mixture distribution over the standard size-threshold measure. Although there was a marginal drop in reliability for C-type granules. The latter is likely due to the low observed genetic variance for C-type granules. Conclusions: The determination of the proportion of granule types from size-distribution is better achieved by using the mixing probabilities from the fit of the mixture distribution rather than using a size-threshold.

Authors


  •   Tanaka, Emi
  •   Ral, Jean-Philippe F. (external author)
  •   Li, Sean (external author)
  •   Gaire, Raj (external author)
  •   Cavanagh, Colin R. (external author)
  •   Cullis, Brian R.
  •   Whan, Alex P. (external author)

Publication Date


  • 2017

Citation


  • Tanaka, E., Ral, J., Li, S., Gaire, R., Cavanagh, C. R., Cullis, B. R. & Whan, A. (2017). Increased accuracy of starch granule type quantification using mixture distributions. Plant Methods, 13 (1), 107-1-107-7.

Scopus Eid


  • 2-s2.0-85037368509

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1904&context=eispapers1

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/902

Start Page


  • 107-1

End Page


  • 107-7

Volume


  • 13

Issue


  • 1

Place Of Publication


  • United Kingdom

Abstract


  • Background: The proportion of granule types in wheat starch is an important characteristic that can affect its functionality. It is widely accepted that granule types are either large, disc-shaped A-type granules or small, spherical B-type granules. Additionally, there are some reports of the tiny C-type granules. The differences between these granule types are due to its carbohydrate composition and crystallinity which is highly, but not perfectly, correlated with the granule size. A majority of the studies that have considered granule types analyse them based on a size threshold rather than chemical composition. This is understandable due to the expense of separating starch into different types. While the use of a size threshold to classify granule type is a low-cost measure, this results in misclassification. We present an alternative, statistical method to quantify the proportion of granule types by a fit of the mixture distribution, along with an R package, a web based app and a video tutorial for how to use the web app to enable its straightforward application. Results: Our results show that the reliability of the gen otypic effects increase approximately 60% using the proportions of the A-type and B-type granule estimated by the mixture distribution over the standard size-threshold measure. Although there was a marginal drop in reliability for C-type granules. The latter is likely due to the low observed genetic variance for C-type granules. Conclusions: The determination of the proportion of granule types from size-distribution is better achieved by using the mixing probabilities from the fit of the mixture distribution rather than using a size-threshold.

Authors


  •   Tanaka, Emi
  •   Ral, Jean-Philippe F. (external author)
  •   Li, Sean (external author)
  •   Gaire, Raj (external author)
  •   Cavanagh, Colin R. (external author)
  •   Cullis, Brian R.
  •   Whan, Alex P. (external author)

Publication Date


  • 2017

Citation


  • Tanaka, E., Ral, J., Li, S., Gaire, R., Cavanagh, C. R., Cullis, B. R. & Whan, A. (2017). Increased accuracy of starch granule type quantification using mixture distributions. Plant Methods, 13 (1), 107-1-107-7.

Scopus Eid


  • 2-s2.0-85037368509

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1904&context=eispapers1

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/902

Start Page


  • 107-1

End Page


  • 107-7

Volume


  • 13

Issue


  • 1

Place Of Publication


  • United Kingdom