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Neural differentiation of induced pluripotent stem cells

Chapter


Abstract


  • The great potential of induced pluripotent cells (iPS) cells is that it allows the possibility of deriving pluripotent stem cells from any human patient. Generation of patient-derived stem cells serves as a great source for developing cell replacement therapies and also for creating human cellular model systems of specific diseases or disorders. This is only of benefit if there are well-established differentiation assay systems to generate the cell types of interest. This chapter describes robust and well-characterized protocols for differentiating iPS cells to neural progenitors, neurons, glia and neural crest cells. These established assays can be applied to iPS cell lines derived from patients with neurodegenerative disorders to study cellular mechanisms associated with neurodegeneration as well as investigating the regenerative potential of patient derived stem cells.

Publication Date


  • 2011

Citation


  • Denham, M. & Dottori, M. (2011). Neural differentiation of induced pluripotent stem cells. In G. Manfredi & H. Kawamata (Eds.), Neurodegeneration: Methods and Protocols (pp. 99-110). New York, United States: Humana Press. http://www.springer.com/gp/book/9781617793271

International Standard Book Number (isbn) 13


  • 9781617793288

Book Title


  • Neurodegeneration: Methods and Protocols

Start Page


  • 99

End Page


  • 110

Place Of Publication


  • New York, United States

Abstract


  • The great potential of induced pluripotent cells (iPS) cells is that it allows the possibility of deriving pluripotent stem cells from any human patient. Generation of patient-derived stem cells serves as a great source for developing cell replacement therapies and also for creating human cellular model systems of specific diseases or disorders. This is only of benefit if there are well-established differentiation assay systems to generate the cell types of interest. This chapter describes robust and well-characterized protocols for differentiating iPS cells to neural progenitors, neurons, glia and neural crest cells. These established assays can be applied to iPS cell lines derived from patients with neurodegenerative disorders to study cellular mechanisms associated with neurodegeneration as well as investigating the regenerative potential of patient derived stem cells.

Publication Date


  • 2011

Citation


  • Denham, M. & Dottori, M. (2011). Neural differentiation of induced pluripotent stem cells. In G. Manfredi & H. Kawamata (Eds.), Neurodegeneration: Methods and Protocols (pp. 99-110). New York, United States: Humana Press. http://www.springer.com/gp/book/9781617793271

International Standard Book Number (isbn) 13


  • 9781617793288

Book Title


  • Neurodegeneration: Methods and Protocols

Start Page


  • 99

End Page


  • 110

Place Of Publication


  • New York, United States