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Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells

Journal Article


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Abstract


  • Adrenomedullary chromaffin cells are catecholamine (CA)-producing cells originating from trunk neural crest (NC) via sympathoadrenal progenitors (SAPs). We generated NC and SAPs from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in vitro via BMP2/FGF2 exposure, ascertained by qPCR and immunoexpression of SOX10, ASCL1, TFAP2a, and PHOX2B, and by fluorescence-activated cell sorting selection for p75NTR and GD2, and confirmed their trunk-like HOX gene expression. We showed that continuing BMP4 and curtailing FGF2 in vitro, augmented with corticosteroid mimetic, induced these cells to upregulate the chromaffin cell-specific marker PNMTand other CA synthesis and storage markers, and we demonstrated noradrenaline and adrenaline by Faglu and high-performance liquid chromatography. We showed these human cells’ SAP-like property of migration and differentiation into cells expressing chromaffin cell markers by implanting them into avian embryos in vivo and in chorio-allantoic membrane grafts. These cells have the potential for investigating differentiation of human chromaffin cells and for modeling diseases involving this cell type.

Authors


  •   Abu-Bonsrah, Kwaku Dad (external author)
  •   Zhang, Dongcheng (external author)
  •   Bjorksten, Andrew R. (external author)
  •   Dottori, Mirella
  •   Newgreen, Donald F. (external author)

Publication Date


  • 2018

Citation


  • Abu-Bonsrah, K., Zhang, D., Bjorksten, A. R., Dottori, M. & Newgreen, D. F. (2018). Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells. Stem Cell Reports, 10 (1), 134-150.

Scopus Eid


  • 2-s2.0-85042779569

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2224&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1197

Number Of Pages


  • 16

Start Page


  • 134

End Page


  • 150

Volume


  • 10

Issue


  • 1

Place Of Publication


  • United States

Abstract


  • Adrenomedullary chromaffin cells are catecholamine (CA)-producing cells originating from trunk neural crest (NC) via sympathoadrenal progenitors (SAPs). We generated NC and SAPs from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in vitro via BMP2/FGF2 exposure, ascertained by qPCR and immunoexpression of SOX10, ASCL1, TFAP2a, and PHOX2B, and by fluorescence-activated cell sorting selection for p75NTR and GD2, and confirmed their trunk-like HOX gene expression. We showed that continuing BMP4 and curtailing FGF2 in vitro, augmented with corticosteroid mimetic, induced these cells to upregulate the chromaffin cell-specific marker PNMTand other CA synthesis and storage markers, and we demonstrated noradrenaline and adrenaline by Faglu and high-performance liquid chromatography. We showed these human cells’ SAP-like property of migration and differentiation into cells expressing chromaffin cell markers by implanting them into avian embryos in vivo and in chorio-allantoic membrane grafts. These cells have the potential for investigating differentiation of human chromaffin cells and for modeling diseases involving this cell type.

Authors


  •   Abu-Bonsrah, Kwaku Dad (external author)
  •   Zhang, Dongcheng (external author)
  •   Bjorksten, Andrew R. (external author)
  •   Dottori, Mirella
  •   Newgreen, Donald F. (external author)

Publication Date


  • 2018

Citation


  • Abu-Bonsrah, K., Zhang, D., Bjorksten, A. R., Dottori, M. & Newgreen, D. F. (2018). Generation of Adrenal Chromaffin-like Cells from Human Pluripotent Stem Cells. Stem Cell Reports, 10 (1), 134-150.

Scopus Eid


  • 2-s2.0-85042779569

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2224&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1197

Number Of Pages


  • 16

Start Page


  • 134

End Page


  • 150

Volume


  • 10

Issue


  • 1

Place Of Publication


  • United States