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Electrophysiological properties of neurosensory progenitors derived from human embryonic stem cells

Journal Article


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Abstract


  • In severe cases of sensorineural hearing loss where the numbers of auditory neurons are significantly depleted, stem cell-derived neurons may provide a potential source of replacement cells. The success of such a therapy relies upon producing a population of functional neurons from stem cells, to enable precise encoding of sound information to the brainstem. Using our established differentiation assay to produce sensory neurons from human stem cells, patch-clamp recordings indicated that all neurons examined generated action potentials and displayed both transient sodium and sustained potassium currents. Stem cell-derived neurons reliably entrained to stimuli up to 20 pulses per second (pps), with 50% entrainment at 50 pps. A comparison with cultured primary auditory neurons indicated similar firing precision during low-frequency stimuli, but significant differences after 50 pps due to differences in action potential latency and width. The firing properties of stem cell-derived neurons were also considered relative to time in culture (31-56. days) and revealed no change in restin g membrane potential, threshold or firing latency over time. Thus, while stem cell-derived neurons did not entrain to high frequency stimulation as effectively as mammalian auditory neurons, their electrical phenotype was stable in culture and consistent with that reported for embryonic auditory neurons.

Authors


  •   Needham, Karina (external author)
  •   Hyakumura, Tomoko (external author)
  •   Gunewardene, Niliksha (external author)
  •   Dottori, Mirella
  •   Nayagam, Bryony A. (external author)

Publication Date


  • 2014

Citation


  • Needham, K., Hyakumura, T., Gunewardene, N., Dottori, M. & Nayagam, B. A. (2014). Electrophysiological properties of neurosensory progenitors derived from human embryonic stem cells. Stem Cell Research, 12 (1), 241-249.

Scopus Eid


  • 2-s2.0-84888229595

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2191&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1164

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 241

End Page


  • 249

Volume


  • 12

Issue


  • 1

Place Of Publication


  • Netherlands

Abstract


  • In severe cases of sensorineural hearing loss where the numbers of auditory neurons are significantly depleted, stem cell-derived neurons may provide a potential source of replacement cells. The success of such a therapy relies upon producing a population of functional neurons from stem cells, to enable precise encoding of sound information to the brainstem. Using our established differentiation assay to produce sensory neurons from human stem cells, patch-clamp recordings indicated that all neurons examined generated action potentials and displayed both transient sodium and sustained potassium currents. Stem cell-derived neurons reliably entrained to stimuli up to 20 pulses per second (pps), with 50% entrainment at 50 pps. A comparison with cultured primary auditory neurons indicated similar firing precision during low-frequency stimuli, but significant differences after 50 pps due to differences in action potential latency and width. The firing properties of stem cell-derived neurons were also considered relative to time in culture (31-56. days) and revealed no change in restin g membrane potential, threshold or firing latency over time. Thus, while stem cell-derived neurons did not entrain to high frequency stimulation as effectively as mammalian auditory neurons, their electrical phenotype was stable in culture and consistent with that reported for embryonic auditory neurons.

Authors


  •   Needham, Karina (external author)
  •   Hyakumura, Tomoko (external author)
  •   Gunewardene, Niliksha (external author)
  •   Dottori, Mirella
  •   Nayagam, Bryony A. (external author)

Publication Date


  • 2014

Citation


  • Needham, K., Hyakumura, T., Gunewardene, N., Dottori, M. & Nayagam, B. A. (2014). Electrophysiological properties of neurosensory progenitors derived from human embryonic stem cells. Stem Cell Research, 12 (1), 241-249.

Scopus Eid


  • 2-s2.0-84888229595

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2191&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1164

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 241

End Page


  • 249

Volume


  • 12

Issue


  • 1

Place Of Publication


  • Netherlands