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WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells

Journal Article


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Abstract


  • Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.

Authors


  •   Alshawaf, Abdullah (external author)
  •   Antonic, Ana (external author)
  •   Skafidas, Efstratios (external author)
  •   Ng, Dominic Chi-Hung (external author)
  •   Dottori, Mirella

Publication Date


  • 2017

Citation


  • Alshawaf, A. J., Antonic, A., Skafidas, E., Ng, D. & Dottori, M. (2017). WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells. Stem Cells International, 2017 7848932-1-7848932-9.

Scopus Eid


  • 2-s2.0-85021658154

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2179&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1153

Has Global Citation Frequency


Start Page


  • 7848932-1

End Page


  • 7848932-9

Volume


  • 2017

Place Of Publication


  • United States

Abstract


  • Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.

Authors


  •   Alshawaf, Abdullah (external author)
  •   Antonic, Ana (external author)
  •   Skafidas, Efstratios (external author)
  •   Ng, Dominic Chi-Hung (external author)
  •   Dottori, Mirella

Publication Date


  • 2017

Citation


  • Alshawaf, A. J., Antonic, A., Skafidas, E., Ng, D. & Dottori, M. (2017). WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells. Stem Cells International, 2017 7848932-1-7848932-9.

Scopus Eid


  • 2-s2.0-85021658154

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2179&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1153

Has Global Citation Frequency


Start Page


  • 7848932-1

End Page


  • 7848932-9

Volume


  • 2017

Place Of Publication


  • United States