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Antimalarial and cytotoxic activities of pregnene-type steroidal alkaloids from Holarrhena pubescens roots

Journal Article


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Abstract


  • The phytochemical investigation of an alkaloidal extract of Holarrhena pubescens roots led to the isolation and identification of a new pregnene-type alkaloid, mokluangin D (1), together with nine known steroidal alkaloids (2–10). The structure of the new metabolite was determined on the basis of spectroscopic analyses including 1D- and 2D-NMR spectroscopy and mass spectrometry. Compounds 3 and 4 showed potent antimalarial activity against Plasmodium falciparum K1 stain with IC 50 values of 1.2 and 2.0 μM, respectively, and showed weak cytotoxic activity against the NCI-H187 cell line with IC 50 values of 27.7 and 30.6 μM, respectively. The substituent groups at C-3 and the carbonyl group at C-18 are important for the activity against the P. falciparum K1 stain.

UOW Authors


  •   Cheenpracha, Sarot (external author)
  •   Boapun, Pannakorn (external author)
  •   Limtharakul, Thunwadee (external author)
  •   Laphookhieo, Surat (external author)
  •   Pyne, Stephen

Publication Date


  • 2017

Citation


  • Cheenpracha, S., Boapun, P., Limtharakul, T., Laphookhieo, S. & Pyne, S. G. (2017). Antimalarial and cytotoxic activities of pregnene-type steroidal alkaloids from Holarrhena pubescens roots. Natural Product Research, Online First 1-7.

Scopus Eid


  • 2-s2.0-85034848291

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6163&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/5110

Number Of Pages


  • 6

Start Page


  • 1

End Page


  • 7

Volume


  • Online First

Place Of Publication


  • United Kingdom

Abstract


  • The phytochemical investigation of an alkaloidal extract of Holarrhena pubescens roots led to the isolation and identification of a new pregnene-type alkaloid, mokluangin D (1), together with nine known steroidal alkaloids (2–10). The structure of the new metabolite was determined on the basis of spectroscopic analyses including 1D- and 2D-NMR spectroscopy and mass spectrometry. Compounds 3 and 4 showed potent antimalarial activity against Plasmodium falciparum K1 stain with IC 50 values of 1.2 and 2.0 μM, respectively, and showed weak cytotoxic activity against the NCI-H187 cell line with IC 50 values of 27.7 and 30.6 μM, respectively. The substituent groups at C-3 and the carbonyl group at C-18 are important for the activity against the P. falciparum K1 stain.

UOW Authors


  •   Cheenpracha, Sarot (external author)
  •   Boapun, Pannakorn (external author)
  •   Limtharakul, Thunwadee (external author)
  •   Laphookhieo, Surat (external author)
  •   Pyne, Stephen

Publication Date


  • 2017

Citation


  • Cheenpracha, S., Boapun, P., Limtharakul, T., Laphookhieo, S. & Pyne, S. G. (2017). Antimalarial and cytotoxic activities of pregnene-type steroidal alkaloids from Holarrhena pubescens roots. Natural Product Research, Online First 1-7.

Scopus Eid


  • 2-s2.0-85034848291

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6163&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/5110

Number Of Pages


  • 6

Start Page


  • 1

End Page


  • 7

Volume


  • Online First

Place Of Publication


  • United Kingdom