This paper presents the development and validation of a simplified nodal model that predicts the vertical temperature profile in a stratum-ventilated room. With full consideration of the mechanism of stratum ventilation and the effects of envelope heat transmission, the vertical temperature profile in a stratum-ventilated room could be predicted. The proposed model was validated through nine experiments of different scenarios. Generally good agreements between the model-predictions and measurements were achieved on the vertical air temperature profile, vertical temperature differences and area-weighted mean surface temperatures of different room enclosures. The agreements were also good for the volume-weighted mean temperatures of the core zone and breathing zone. Therefore, this nodal model can be used for energy calculation and practical engineering design.