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Diatrack particle tracking software: Review of applications and performance evaluation

Journal Article


Abstract


  • Object tracking is an instrumental tool supporting studies of cellular trafficking. There are three challenges in object tracking: the identification of targets; the precise determination of their position and boundaries; and the assembly of correct trajectories. This last challenge is particularly relevant when dealing with densely populated images with low signal-to-noise ratios—conditions that are often encountered in applications such as organelle tracking, virus particle tracking or single-molecule imaging. We have developed a set of methods that can handle a wide variety of signal complexities. They are compiled into a free software package called Diatrack. Here we review its main features and utility in a range of applications, providing a survey of the dynamic imaging field together with recommendations for effective use. The performance of our framework is shown to compare favorably to a wide selection of custom-developed algorithms, whether in terms of localization precision, processing speed or correctness of tracks.

Authors


  •   Vallotton, Pascal (external author)
  •   van Oijen, Antoine M.
  •   Whitchurch, Cynthia B. (external author)
  •   Gelfand, Vladimir (external author)
  •   Yeo, Leslie (external author)
  •   Tsiavaliaris, Georgios (external author)
  •   Heinrich, Stephanie (external author)
  •   Dultz, Elisa (external author)
  •   Weis, Karsten (external author)
  •   Grünwald, David (external author)

Publication Date


  • 2017

Citation


  • Vallotton, P., van Oijen, A. M., Whitchurch, C. B., Gelfand, V., Yeo, L., Tsiavaliaris, G., Heinrich, S., Dultz, E., Weis, K. & Grünwald, D. (2017). Diatrack particle tracking software: Review of applications and performance evaluation. Traffic, 18 (12), 840-852.

Scopus Eid


  • 2-s2.0-85033363906

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/5052

Number Of Pages


  • 12

Start Page


  • 840

End Page


  • 852

Volume


  • 18

Issue


  • 12

Place Of Publication


  • United Kingdom

Abstract


  • Object tracking is an instrumental tool supporting studies of cellular trafficking. There are three challenges in object tracking: the identification of targets; the precise determination of their position and boundaries; and the assembly of correct trajectories. This last challenge is particularly relevant when dealing with densely populated images with low signal-to-noise ratios—conditions that are often encountered in applications such as organelle tracking, virus particle tracking or single-molecule imaging. We have developed a set of methods that can handle a wide variety of signal complexities. They are compiled into a free software package called Diatrack. Here we review its main features and utility in a range of applications, providing a survey of the dynamic imaging field together with recommendations for effective use. The performance of our framework is shown to compare favorably to a wide selection of custom-developed algorithms, whether in terms of localization precision, processing speed or correctness of tracks.

Authors


  •   Vallotton, Pascal (external author)
  •   van Oijen, Antoine M.
  •   Whitchurch, Cynthia B. (external author)
  •   Gelfand, Vladimir (external author)
  •   Yeo, Leslie (external author)
  •   Tsiavaliaris, Georgios (external author)
  •   Heinrich, Stephanie (external author)
  •   Dultz, Elisa (external author)
  •   Weis, Karsten (external author)
  •   Grünwald, David (external author)

Publication Date


  • 2017

Citation


  • Vallotton, P., van Oijen, A. M., Whitchurch, C. B., Gelfand, V., Yeo, L., Tsiavaliaris, G., Heinrich, S., Dultz, E., Weis, K. & Grünwald, D. (2017). Diatrack particle tracking software: Review of applications and performance evaluation. Traffic, 18 (12), 840-852.

Scopus Eid


  • 2-s2.0-85033363906

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/5052

Number Of Pages


  • 12

Start Page


  • 840

End Page


  • 852

Volume


  • 18

Issue


  • 12

Place Of Publication


  • United Kingdom