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Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody

Journal Article


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Abstract


  • The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold-loaded nanoporous ferric oxide nanocubes (Au–NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked eye) detection of autoantibodies. The results showed that Au–NPFe2O3NC exhibits enhanced peroxidase-like activity toward the catalytic oxidation of 3,3′,5,5′-tertamethylbenzidine (TMB) in the presence of H2O2 at room temperature (25 °C) and follows the typical Michaelis–Menten kinetics. The autoantibody sensor based on this intrinsic property of Au–NPFe2O3NC resulted in excellent detection sensitivity [limit of detection (LOD) = 0.08 U/mL] and reproducibility [percent relative standard deviation (% RSD) = <5% for n = 3] for analyzing p53-specific autoantibodies using electrochemical and colorimetric (naked eye) readouts. The clinical applicability of the sensor has been tested in detecting p53-specific autoantibody in plasma obtained from patients with epithelial ovarian cancer high-grade serous subtype (EOCHGS, number of samples = 2) and controls (benign, number of samples = 2). As Au–NPFe2O3NC possess high peroxidase-like activity for the oxidation of TMB in the presence of H2O2 [TMB is a common chromogenic substrate for HRP in enzyme-linked immunosorbent assays (ELISAs)], we envisage that our assay could find a wide range of application in developing ELISA-based sensing approaches in the fields of medicine (i.e., detection of other biomarkers the same as p53 autoantibody), biotechnology, and environmental sciences.

UOW Authors


  •   Masud, Mostafa (external author)
  •   Yadav, Sharda (external author)
  •   Islam, Md Nazmul (external author)
  •   Nguyen, Nam-Trung (external author)
  •   Salomon, Carlos (external author)
  •   Kline, Richard (external author)
  •   Alamri, Hatem (external author)
  •   Alothman, Zeid Abdullah. (external author)
  •   Yamauchi, Yusuke (external author)
  •   Hossain, Md Shahriar
  •   Shiddiky, Muhammad J. A. (external author)

Publication Date


  • 2017

Citation


  • Masud, M. Kamal., Yadav, S., Islam, M., Nguyen, N., Salomon, C., Kline, R., Alamri, H. R., Alothman, Z. A., Yamauchi, Y., Hossain, M. A. & Shiddiky, M. J. A. (2017). Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody. Analytical Chemistry, 89 (20), 11005-11013.

Scopus Eid


  • 2-s2.0-85031421742

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3823&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2773

Number Of Pages


  • 8

Start Page


  • 11005

End Page


  • 11013

Volume


  • 89

Issue


  • 20

Place Of Publication


  • United States

Abstract


  • The enzyme-mimicking activity of iron oxide based nanostructures has provided a significant advantage in developing advanced molecular sensors for biomedical and environmental applications. Herein, we introduce the horseradish peroxidase (HRP)-like activity of gold-loaded nanoporous ferric oxide nanocubes (Au–NPFe2O3NC) for the development of a molecular sensor with enhanced electrocatalytic and colorimetric (naked eye) detection of autoantibodies. The results showed that Au–NPFe2O3NC exhibits enhanced peroxidase-like activity toward the catalytic oxidation of 3,3′,5,5′-tertamethylbenzidine (TMB) in the presence of H2O2 at room temperature (25 °C) and follows the typical Michaelis–Menten kinetics. The autoantibody sensor based on this intrinsic property of Au–NPFe2O3NC resulted in excellent detection sensitivity [limit of detection (LOD) = 0.08 U/mL] and reproducibility [percent relative standard deviation (% RSD) = <5% for n = 3] for analyzing p53-specific autoantibodies using electrochemical and colorimetric (naked eye) readouts. The clinical applicability of the sensor has been tested in detecting p53-specific autoantibody in plasma obtained from patients with epithelial ovarian cancer high-grade serous subtype (EOCHGS, number of samples = 2) and controls (benign, number of samples = 2). As Au–NPFe2O3NC possess high peroxidase-like activity for the oxidation of TMB in the presence of H2O2 [TMB is a common chromogenic substrate for HRP in enzyme-linked immunosorbent assays (ELISAs)], we envisage that our assay could find a wide range of application in developing ELISA-based sensing approaches in the fields of medicine (i.e., detection of other biomarkers the same as p53 autoantibody), biotechnology, and environmental sciences.

UOW Authors


  •   Masud, Mostafa (external author)
  •   Yadav, Sharda (external author)
  •   Islam, Md Nazmul (external author)
  •   Nguyen, Nam-Trung (external author)
  •   Salomon, Carlos (external author)
  •   Kline, Richard (external author)
  •   Alamri, Hatem (external author)
  •   Alothman, Zeid Abdullah. (external author)
  •   Yamauchi, Yusuke (external author)
  •   Hossain, Md Shahriar
  •   Shiddiky, Muhammad J. A. (external author)

Publication Date


  • 2017

Citation


  • Masud, M. Kamal., Yadav, S., Islam, M., Nguyen, N., Salomon, C., Kline, R., Alamri, H. R., Alothman, Z. A., Yamauchi, Y., Hossain, M. A. & Shiddiky, M. J. A. (2017). Gold-Loaded Nanoporous Ferric Oxide Nanocubes with Peroxidase-Mimicking Activity for Electrocatalytic and Colorimetric Detection of Autoantibody. Analytical Chemistry, 89 (20), 11005-11013.

Scopus Eid


  • 2-s2.0-85031421742

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3823&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2773

Number Of Pages


  • 8

Start Page


  • 11005

End Page


  • 11013

Volume


  • 89

Issue


  • 20

Place Of Publication


  • United States