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In situ handheld three-dimensional bioprinting for cartilage regeneration

Journal Article


Abstract


  • Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ three-dimensional (3D) printing is an exciting and innovative biofabrication technology that enables the surgeon to deliver tissue-engineering techniques at the time and location of need. We have created a hand-held 3D printing device (biopen) that allows the simultaneous coaxial extrusion of bioscaffold and cultured cells directly into the cartilage defect in vivo in a single-session surgery. This pilot study assessed the ability of the biopen to repair a full-thickness chondral defect and the early outcomes in cartilage regeneration, and compared these results with other treatments in a large animal model. A standardized critical-sized full-thickness chondral defect was created in the weight-bearing surface of the lateral and medial condyles of both femurs of six sheep. Each defect was treated with one of the following treatments: (i) hand-held in situ 3D printed bioscaffold using the biopen (HH group), (ii) preconstructed bench-based printed bioscaffolds (BB group), (iii) microfractures (MF group) or (iv) untreated (control, C group). At 8 weeks after surgery, macroscopic, microscopic and biomechanical tests were performed. Surgical 3D bioprinting was performed in all animals without any intra- or postoperative complication. The HH biopen allowed early cartilage regeneration. The results of this study show that real-time, in vivo bioprinting with cells and scaffold is a feasible means of delivering a regenerative medicine strategy in a large animal model to regenerate articular cartilage.

Authors


  •   Di Bella, Claudia (external author)
  •   Duchi, Serena (external author)
  •   O'Connell, Cathal (external author)
  •   Blanchard, Romane (external author)
  •   Augustine, Cheryl (external author)
  •   Yue, Zhilian
  •   Thompson, Fletcher W. (external author)
  •   Richards, Christopher J.
  •   Beirne, Stephen T.
  •   Onofrillo, Carmine (external author)
  •   Bauquier, Sebastien (external author)
  •   Ryan, Stewart D. (external author)
  •   Pivonka, Peter (external author)
  •   Wallace, Gordon G.
  •   Choong, Peter (external author)

Publication Date


  • 2018

Citation


  • Di Bella, C., Duchi, S., O'Connell, C. D., Blanchard, R., Augustine, C., Yue, Z., Thompson, F., Richards, C., Beirne, S., Onofrillo, C., Bauquier, S. H., Ryan, S. D., Pivonka, P., Wallace, G. G. & Choong, P. F. (2018). In situ handheld three-dimensional bioprinting for cartilage regeneration. Journal of Tissue Engineering and Regenerative Medicine, 12 (3), 611-621.

Scopus Eid


  • 2-s2.0-85028297987

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2735

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 611

End Page


  • 621

Volume


  • 12

Issue


  • 3

Place Of Publication


  • United States

Abstract


  • Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ three-dimensional (3D) printing is an exciting and innovative biofabrication technology that enables the surgeon to deliver tissue-engineering techniques at the time and location of need. We have created a hand-held 3D printing device (biopen) that allows the simultaneous coaxial extrusion of bioscaffold and cultured cells directly into the cartilage defect in vivo in a single-session surgery. This pilot study assessed the ability of the biopen to repair a full-thickness chondral defect and the early outcomes in cartilage regeneration, and compared these results with other treatments in a large animal model. A standardized critical-sized full-thickness chondral defect was created in the weight-bearing surface of the lateral and medial condyles of both femurs of six sheep. Each defect was treated with one of the following treatments: (i) hand-held in situ 3D printed bioscaffold using the biopen (HH group), (ii) preconstructed bench-based printed bioscaffolds (BB group), (iii) microfractures (MF group) or (iv) untreated (control, C group). At 8 weeks after surgery, macroscopic, microscopic and biomechanical tests were performed. Surgical 3D bioprinting was performed in all animals without any intra- or postoperative complication. The HH biopen allowed early cartilage regeneration. The results of this study show that real-time, in vivo bioprinting with cells and scaffold is a feasible means of delivering a regenerative medicine strategy in a large animal model to regenerate articular cartilage.

Authors


  •   Di Bella, Claudia (external author)
  •   Duchi, Serena (external author)
  •   O'Connell, Cathal (external author)
  •   Blanchard, Romane (external author)
  •   Augustine, Cheryl (external author)
  •   Yue, Zhilian
  •   Thompson, Fletcher W. (external author)
  •   Richards, Christopher J.
  •   Beirne, Stephen T.
  •   Onofrillo, Carmine (external author)
  •   Bauquier, Sebastien (external author)
  •   Ryan, Stewart D. (external author)
  •   Pivonka, Peter (external author)
  •   Wallace, Gordon G.
  •   Choong, Peter (external author)

Publication Date


  • 2018

Citation


  • Di Bella, C., Duchi, S., O'Connell, C. D., Blanchard, R., Augustine, C., Yue, Z., Thompson, F., Richards, C., Beirne, S., Onofrillo, C., Bauquier, S. H., Ryan, S. D., Pivonka, P., Wallace, G. G. & Choong, P. F. (2018). In situ handheld three-dimensional bioprinting for cartilage regeneration. Journal of Tissue Engineering and Regenerative Medicine, 12 (3), 611-621.

Scopus Eid


  • 2-s2.0-85028297987

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2735

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 611

End Page


  • 621

Volume


  • 12

Issue


  • 3

Place Of Publication


  • United States