placeholder image

Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

Journal Article


Abstract


  • Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue

    engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a

    semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction

    that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully

    addressed to generate a structure stiff enough to withstand the forces phisiologically applied on

    articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently

    developed a handheld 3D printer called “Biopen”. To progress towards translating this freeform

    biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would

    deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill

    those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system

    allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved

    after only 10seconds of exposure to 700mW/cm2 of 365nm UV-A, containing >90% viable stem cells

    that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled

    rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical

    cartilage engineering.

Authors


  •   Duchi, Serena (external author)
  •   Onofrillo, Carmine (external author)
  •   O'Connell, Cathal (external author)
  •   Blanchard, Romane (external author)
  •   Augustine, Cheryl (external author)
  •   Quigley, Anita F.
  •   Kapsa, Robert M. I.
  •   Pivonka, Peter (external author)
  •   Wallace, Gordon G.
  •   Di Bella, Claudia (external author)
  •   Choong, Peter F. M. (external author)

Publication Date


  • 2017

Citation


  • Duchi, S., Onofrillo, C., O'Connell, C. D., Blanchard, R., Augustine, C., Quigley, A. F., Kapsa, R. M. I., Pivonka, P., Wallace, G., Di Bella, C. & Choong, P. F. M. (2017). Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair. Scientific Reports, 7 5837-1-5837-12.

Scopus Eid


  • 2-s2.0-85025660408

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2651

Start Page


  • 5837-1

End Page


  • 5837-12

Volume


  • 7

Place Of Publication


  • United Kingdom

Abstract


  • Three-dimensional (3D) bioprinting is driving major innovations in the area of cartilage tissue

    engineering. Extrusion-based 3D bioprinting necessitates a phase change from a liquid bioink to a

    semi-solid crosslinked network achieved by a photo-initiated free radical polymerization reaction

    that is known to be cytotoxic. Therefore, the choice of the photocuring conditions has to be carefully

    addressed to generate a structure stiff enough to withstand the forces phisiologically applied on

    articular cartilage, while ensuring adequate cell survival for functional chondral repair. We recently

    developed a handheld 3D printer called “Biopen”. To progress towards translating this freeform

    biofabrication tool into clinical practice, we aimed to define the ideal bioprinting conditions that would

    deliver a scaffold with high cell viability and structural stiffness relevant for chondral repair. To fulfill

    those criteria, free radical cytotoxicity was confined by a co-axial Core/Shell separation. This system

    allowed the generation of Core/Shell GelMa/HAMa bioscaffolds with stiffness of 200KPa, achieved

    after only 10seconds of exposure to 700mW/cm2 of 365nm UV-A, containing >90% viable stem cells

    that retained proliferative capacity. Overall, the Core/Shell handheld 3D bioprinting strategy enabled

    rapid generation of high modulus bioscaffolds with high cell viability, with potential for in situ surgical

    cartilage engineering.

Authors


  •   Duchi, Serena (external author)
  •   Onofrillo, Carmine (external author)
  •   O'Connell, Cathal (external author)
  •   Blanchard, Romane (external author)
  •   Augustine, Cheryl (external author)
  •   Quigley, Anita F.
  •   Kapsa, Robert M. I.
  •   Pivonka, Peter (external author)
  •   Wallace, Gordon G.
  •   Di Bella, Claudia (external author)
  •   Choong, Peter F. M. (external author)

Publication Date


  • 2017

Citation


  • Duchi, S., Onofrillo, C., O'Connell, C. D., Blanchard, R., Augustine, C., Quigley, A. F., Kapsa, R. M. I., Pivonka, P., Wallace, G., Di Bella, C. & Choong, P. F. M. (2017). Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair. Scientific Reports, 7 5837-1-5837-12.

Scopus Eid


  • 2-s2.0-85025660408

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2651

Start Page


  • 5837-1

End Page


  • 5837-12

Volume


  • 7

Place Of Publication


  • United Kingdom