This paper reports the development of a nonenzymatic, amplificationfree,
and sensitive platform for the detection of microRNA based on a
new class of electrocatalytically active superparamagnetic gold-loaded
nanoporous iron oxide nanocubes (Au@NPFe2O3NC). The assay showed
an excellent detection sensitivity down to 100 fM and specificity towards
the analysis of miR-21 in cell lines and tissue samples derived from
patients with oesophageal squamous-cell carcinoma (ESCC).