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The effect of antioxidants on sperm motility activation in the Booroolong frog

Journal Article


Abstract


  • Motile sperm can generate high levels of reactive oxygen species (ROS) post activation, and ROS can quickly accumulate to levels that impair motility and fertilising ability. The addition of antioxidants to sperm suspensions has been suggested as a means of reducing oxidative stress and enhancing sperm motility during and after sperm storage. Despite this, very few studies have attempted to experimentally test the effects of antioxidants on sperm motility activation in animals that use an external mode of fertilisation, espcially in amphibians. The present study aimed to investigate the effect of vitamin C and vitamin E on sperm motility activation in the Booroolong frog. Spermatozoa were activated in media containing either vitamin C (0, 0.05, 0.10, 0.15, 0.20, 0.25¿g¿L-1) or vitamin E (0, 0.25, 0.50, 0.75, 1.0, 1.25 1.50, 1.75¿g¿L-1). Sperm performance parameters (percent motility and velocity) were assessed using CASA at 0, 1, 2, 3, 4, 5 and 6h post-activation. Contrary to expectations, vitamin C supplementation was detrimental to sperm motility across all tested concentrations, while vitamin E had no effect. Further investigation on the endogenous antioxidant system of anuran sperm is required to ascertain whether alternative antioxidants may be more suitable at reducing ROS produced during sperm activation and improving sperm motility activation in vitro.

Publication Date


  • 2017

Citation


  • Keogh, L. M., Byrne, P. G. & Silla, A. J. (2017). The effect of antioxidants on sperm motility activation in the Booroolong frog. Animal Reproduction Science, 183 126-131.

Scopus Eid


  • 2-s2.0-85020208245

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4858

Number Of Pages


  • 5

Start Page


  • 126

End Page


  • 131

Volume


  • 183

Place Of Publication


  • Netherlands

Abstract


  • Motile sperm can generate high levels of reactive oxygen species (ROS) post activation, and ROS can quickly accumulate to levels that impair motility and fertilising ability. The addition of antioxidants to sperm suspensions has been suggested as a means of reducing oxidative stress and enhancing sperm motility during and after sperm storage. Despite this, very few studies have attempted to experimentally test the effects of antioxidants on sperm motility activation in animals that use an external mode of fertilisation, espcially in amphibians. The present study aimed to investigate the effect of vitamin C and vitamin E on sperm motility activation in the Booroolong frog. Spermatozoa were activated in media containing either vitamin C (0, 0.05, 0.10, 0.15, 0.20, 0.25¿g¿L-1) or vitamin E (0, 0.25, 0.50, 0.75, 1.0, 1.25 1.50, 1.75¿g¿L-1). Sperm performance parameters (percent motility and velocity) were assessed using CASA at 0, 1, 2, 3, 4, 5 and 6h post-activation. Contrary to expectations, vitamin C supplementation was detrimental to sperm motility across all tested concentrations, while vitamin E had no effect. Further investigation on the endogenous antioxidant system of anuran sperm is required to ascertain whether alternative antioxidants may be more suitable at reducing ROS produced during sperm activation and improving sperm motility activation in vitro.

Publication Date


  • 2017

Citation


  • Keogh, L. M., Byrne, P. G. & Silla, A. J. (2017). The effect of antioxidants on sperm motility activation in the Booroolong frog. Animal Reproduction Science, 183 126-131.

Scopus Eid


  • 2-s2.0-85020208245

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4858

Number Of Pages


  • 5

Start Page


  • 126

End Page


  • 131

Volume


  • 183

Place Of Publication


  • Netherlands