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Derivation of Human Embryonic Stem Cells

Chapter


Abstract


  • Embryonic stem cells (ESCs) represent a mainstay for pluripotent stem cell research and development (R&D) and provide tangible opportunities for clinical translation including cell therapies and drug discovery. Moreover, in spite of the discovery of induced pluripotent stem cells (iPSCs), ESCs are an essential reference point, against which other pluripotent cells are compared. Hence, there is an ongoing need to derive and bank quality-controlled research-grade and clinical-grade ESC lines using established and standardized methods. Here, we provide a concise, step-by-step protocol for the derivation of ESCs from human embryos. While largely based on previously reported method for clinical-grade human ESC (hESC) line derivation, the protocol is suitable for routine application, although adaptable for clinical-compliance.

Authors


  •   Crook, Jeremy M.
  •   Kravets, Lucy (external author)
  •   Peura, Teija (external author)
  •   Firpo, Meri (external author)

Publication Date


  • 2017

Citation


  • Crook, J. M., Kravets, L., Peura, T. & Firpo, M. T. (2017). Derivation of Human Embryonic Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 115-129). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016988546

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2609

Book Title


  • Stem Cell Banking: Concepts and Protocols

Start Page


  • 115

End Page


  • 129

Place Of Publication


  • New York, United States

Abstract


  • Embryonic stem cells (ESCs) represent a mainstay for pluripotent stem cell research and development (R&D) and provide tangible opportunities for clinical translation including cell therapies and drug discovery. Moreover, in spite of the discovery of induced pluripotent stem cells (iPSCs), ESCs are an essential reference point, against which other pluripotent cells are compared. Hence, there is an ongoing need to derive and bank quality-controlled research-grade and clinical-grade ESC lines using established and standardized methods. Here, we provide a concise, step-by-step protocol for the derivation of ESCs from human embryos. While largely based on previously reported method for clinical-grade human ESC (hESC) line derivation, the protocol is suitable for routine application, although adaptable for clinical-compliance.

Authors


  •   Crook, Jeremy M.
  •   Kravets, Lucy (external author)
  •   Peura, Teija (external author)
  •   Firpo, Meri (external author)

Publication Date


  • 2017

Citation


  • Crook, J. M., Kravets, L., Peura, T. & Firpo, M. T. (2017). Derivation of Human Embryonic Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 115-129). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016988546

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2609

Book Title


  • Stem Cell Banking: Concepts and Protocols

Start Page


  • 115

End Page


  • 129

Place Of Publication


  • New York, United States