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Cryobanking Pluripotent Stem Cells

Chapter


Abstract


  • Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

Publication Date


  • 2017

Citation


  • Crook, J. M., Tomaskovic-Crook, E. & Ludwig, T. E. (2017). Cryobanking Pluripotent Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 151-164). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016940287

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2608

Book Title


  • Stem Cell Banking: Concepts and Protocols

Start Page


  • 151

End Page


  • 164

Place Of Publication


  • New York, United States

Abstract


  • Cryobanking human pluripotent stem cells (hPSCs), be they human embryonic (hESCs) or induced pluripotent stem cells (iPSCs), is essential for their use in research and cell-based therapeutics. Working and master cell banks can be generated with a desired level of quality assurance applied during cell freezing and storage. Conventional vitrification has evolved to more advanced control rate freezing, culminating in a myriad of published protocols with variable proficiencies and clinical efficacies. Notwithstanding, standardized and reliable protocols are necessary for basic science through to applied research and clinical product development. This chapter details several methods for hPSC cryopreservation, suitable for routine application, high-quality research, and adaptable for clinical compliance.

Publication Date


  • 2017

Citation


  • Crook, J. M., Tomaskovic-Crook, E. & Ludwig, T. E. (2017). Cryobanking Pluripotent Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 151-164). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016940287

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2608

Book Title


  • Stem Cell Banking: Concepts and Protocols

Start Page


  • 151

End Page


  • 164

Place Of Publication


  • New York, United States