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Culturing and Cryobanking Human Neural Stem Cells

Chapter


Abstract


  • The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

Publication Date


  • 2017

Citation


  • Crook, J. M. & Tomaskovic-Crook, E. (2017). Culturing and Cryobanking Human Neural Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 199-206). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016929950

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2607

Book Title


  • Stem Cell Banking: Concepts and Protocols

Has Global Citation Frequency


Start Page


  • 199

End Page


  • 206

Place Of Publication


  • New York, United States

Abstract


  • The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

Publication Date


  • 2017

Citation


  • Crook, J. M. & Tomaskovic-Crook, E. (2017). Culturing and Cryobanking Human Neural Stem Cells. In J. M. Crook & T. E. Ludwig (Eds.), Stem Cell Banking: Concepts and Protocols (pp. 199-206). New York, United States: Humana Press.

International Standard Book Number (isbn) 13


  • 9781493969210

Scopus Eid


  • 2-s2.0-85016929950

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2607

Book Title


  • Stem Cell Banking: Concepts and Protocols

Has Global Citation Frequency


Start Page


  • 199

End Page


  • 206

Place Of Publication


  • New York, United States