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Electrochemical biosensing strategies for DNA methylation analysis

Journal Article


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Abstract


  • DNA methylation is one of the key epigenetic modifications of DNA that results from the enzymatic addition of a methyl group at the fifth carbon of the cytosine base. It plays a crucial role in cellular development, genomic stability and gene expression. Aberrant DNA methylation is responsible for the pathogenesis of many diseases including cancers. Over the past several decades, many methodologies have been developed to detect DNA methylation. These methodologies range from classical molecular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the more recent electrochemical approaches. Among these, electrochemical approaches offer sensitive, simple, specific, rapid, and cost-effective analysis of DNA methylation. Additionally, electrochemical methods are highly amenable to miniaturization and possess the potential to be multiplexed. In recent years, several reviews have provided information on the detection strategies of DNA methylation. However, to date, there is no comprehensive evaluation of electrochemical DNA methylation detection strategies. Herein, we address the recent developments of electrochemical DNA methylation detection approaches. Furthermore, we highlight the major technical and biological challenges involved in these strategies and provide suggestions for the future direction of this important field.

Authors


  •   Hossain, Tanvir (external author)
  •   Mahmudunnabi, Golam (external author)
  •   Masud, Mostafa (external author)
  •   Islam, Md Nazmul (external author)
  •   Ooi, Lezanne
  •   Konstantinov, Konstantin K.
  •   Hossain, Md Shahriar
  •   Martinac, Boris (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Shiddiky, Muhammad J. A. (external author)

Publication Date


  • 2017

Citation


  • Hossain, T., Mahmudunnabi, G., Masud, M. Kamal., Islam, M., Ooi, L., Konstantinov, K., Hossain, M., Martinac, B., Alici, G., Nguyen, N. & Shiddiky, M. J. A. (2017). Electrochemical biosensing strategies for DNA methylation analysis. Biosensors and Bioelectronics, 94 63-73.

Scopus Eid


  • 2-s2.0-85014042313

Ro Full-text Url


  • http://ro.uow.edu.au/context/aiimpapers/article/3460/type/native/viewcontent

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2437

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 63

End Page


  • 73

Volume


  • 94

Place Of Publication


  • Netherlands

Abstract


  • DNA methylation is one of the key epigenetic modifications of DNA that results from the enzymatic addition of a methyl group at the fifth carbon of the cytosine base. It plays a crucial role in cellular development, genomic stability and gene expression. Aberrant DNA methylation is responsible for the pathogenesis of many diseases including cancers. Over the past several decades, many methodologies have been developed to detect DNA methylation. These methodologies range from classical molecular biology and optical approaches, such as bisulfite sequencing, microarrays, quantitative real-time PCR, colorimetry, Raman spectroscopy to the more recent electrochemical approaches. Among these, electrochemical approaches offer sensitive, simple, specific, rapid, and cost-effective analysis of DNA methylation. Additionally, electrochemical methods are highly amenable to miniaturization and possess the potential to be multiplexed. In recent years, several reviews have provided information on the detection strategies of DNA methylation. However, to date, there is no comprehensive evaluation of electrochemical DNA methylation detection strategies. Herein, we address the recent developments of electrochemical DNA methylation detection approaches. Furthermore, we highlight the major technical and biological challenges involved in these strategies and provide suggestions for the future direction of this important field.

Authors


  •   Hossain, Tanvir (external author)
  •   Mahmudunnabi, Golam (external author)
  •   Masud, Mostafa (external author)
  •   Islam, Md Nazmul (external author)
  •   Ooi, Lezanne
  •   Konstantinov, Konstantin K.
  •   Hossain, Md Shahriar
  •   Martinac, Boris (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Shiddiky, Muhammad J. A. (external author)

Publication Date


  • 2017

Citation


  • Hossain, T., Mahmudunnabi, G., Masud, M. Kamal., Islam, M., Ooi, L., Konstantinov, K., Hossain, M., Martinac, B., Alici, G., Nguyen, N. & Shiddiky, M. J. A. (2017). Electrochemical biosensing strategies for DNA methylation analysis. Biosensors and Bioelectronics, 94 63-73.

Scopus Eid


  • 2-s2.0-85014042313

Ro Full-text Url


  • http://ro.uow.edu.au/context/aiimpapers/article/3460/type/native/viewcontent

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2437

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 63

End Page


  • 73

Volume


  • 94

Place Of Publication


  • Netherlands