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Electrical stimulation using conductive polymer polypyrrole counters reduced neurite outgrowth of primary prefrontal cortical neurons from NRG1-KO and DISC1-LI mice

Journal Article


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Abstract


  • Deficits in neurite outgrowth, possibly involving dysregulation of risk genes neuregulin-1 (NRG1)

    and disrupted in schizophrenia 1 (DISC1) have been implicated in psychiatric disorders including

    schizophrenia. Electrical stimulation using conductive polymers has been shown to stimulate

    neurite outgrowth of differentiating human neural stem cells. This study investigated the use of the

    electroactive conductive polymer polypyrrole (Ppy) to counter impaired neurite outgrowth of primary

    pre-frontal cortical (PFC) neurons from NRG1-knock out (NRG1-KO) and DISC1-locus impairment

    (DISC1-LI) mice. Whereas NRG1-KO and DISC1-LI exhibited reduced neurite length and number of

    neurite branches compared to wild-type controls, this was not apparent for cultures on electroactive

    Ppy. Additionally, the use of the Ppy substrate normalised the synaptophysin and PSD95 protein and

    mRNA expression whereas both are usually reduced by NRG1-KO or DISC1-LI. Our findings support the

    utility of Ppy mediated electrical stimulation to prevent the reduction of neurite outgrowth and related

    synaptic protein expression in the primary PFC neurons from NRG1-KO and DISC1-LI mice, providing

    proof-of-concept for treating neurodevelopmental diseases including schizophrenia.

Publication Date


  • 2017

Citation


  • Zhang, Q., Esrafilzadeh, D., Crook, J. M., Kapsa, R., Stewart, E. M., Tomaskovic-Crook, E., Wallace, G. G. & Huang, X. (2017). Electrical stimulation using conductive polymer polypyrrole counters reduced neurite outgrowth of primary prefrontal cortical neurons from NRG1-KO and DISC1-LI mice. Scientific Reports, 7 42525 -1-42525 -8.

Scopus Eid


  • 2-s2.0-85012991907

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3375&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2352

Start Page


  • 42525 -1

End Page


  • 42525 -8

Volume


  • 7

Place Of Publication


  • United Kingdom

Abstract


  • Deficits in neurite outgrowth, possibly involving dysregulation of risk genes neuregulin-1 (NRG1)

    and disrupted in schizophrenia 1 (DISC1) have been implicated in psychiatric disorders including

    schizophrenia. Electrical stimulation using conductive polymers has been shown to stimulate

    neurite outgrowth of differentiating human neural stem cells. This study investigated the use of the

    electroactive conductive polymer polypyrrole (Ppy) to counter impaired neurite outgrowth of primary

    pre-frontal cortical (PFC) neurons from NRG1-knock out (NRG1-KO) and DISC1-locus impairment

    (DISC1-LI) mice. Whereas NRG1-KO and DISC1-LI exhibited reduced neurite length and number of

    neurite branches compared to wild-type controls, this was not apparent for cultures on electroactive

    Ppy. Additionally, the use of the Ppy substrate normalised the synaptophysin and PSD95 protein and

    mRNA expression whereas both are usually reduced by NRG1-KO or DISC1-LI. Our findings support the

    utility of Ppy mediated electrical stimulation to prevent the reduction of neurite outgrowth and related

    synaptic protein expression in the primary PFC neurons from NRG1-KO and DISC1-LI mice, providing

    proof-of-concept for treating neurodevelopmental diseases including schizophrenia.

Publication Date


  • 2017

Citation


  • Zhang, Q., Esrafilzadeh, D., Crook, J. M., Kapsa, R., Stewart, E. M., Tomaskovic-Crook, E., Wallace, G. G. & Huang, X. (2017). Electrical stimulation using conductive polymer polypyrrole counters reduced neurite outgrowth of primary prefrontal cortical neurons from NRG1-KO and DISC1-LI mice. Scientific Reports, 7 42525 -1-42525 -8.

Scopus Eid


  • 2-s2.0-85012991907

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3375&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2352

Start Page


  • 42525 -1

End Page


  • 42525 -8

Volume


  • 7

Place Of Publication


  • United Kingdom