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Design and engineering of water-soluble light-harvesting protein maquettes

Journal Article


Abstract


  • Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting

    and energy capture in light-induced charge separation. By designing and creating nanometer scale

    artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and

    overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor

    efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole

    cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for

    cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of

    the basic physical chemistry of protein and cofactors that drive nanometer scale self-assembly.

    Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid

    natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning

    into the non-polar core of a man-made water soluble protein designed according to elementary first

    principles of protein folding. This partitioning is followed by site-specific anchoring of tetrapyrroles to

    histidine ligands strategically placed for design control of rates and efficiencies of light energy and

    electron transfer while orienting at least one polar group towards the aqueous phase

Authors


  •   Kodali, Goutham (external author)
  •   Mancini, Joshua A. (external author)
  •   Solomon, Lee A. (external author)
  •   Episova, Tatiana V. (external author)
  •   Roach, Nicholas (external author)
  •   Hobbs, Christopher (external author)
  •   Wagner, Pawel
  •   Mass, Olga (external author)
  •   Aravindu, Kunche (external author)
  •   Barnsley, Jonathan E. (external author)
  •   Gordon, Keith C. (external author)
  •   Officer, David L.
  •   Dutton, P. Leslie (external author)
  •   Moser, Christopher C. (external author)

Publication Date


  • 2017

Citation


  • Kodali, G., Mancini, J. A., Solomon, L. A., Episova, T., Roach, N., Hobbs, C., Wagner, P. W., Mass, O., Aravindu, K., Barnsley, J. E., Gordon, K. C., Officer, D. L., Dutton, P. & Moser, C. C. (2017). Design and engineering of water-soluble light-harvesting protein maquettes. Chemical Science, 8 316-324.

Scopus Eid


  • 2-s2.0-85007150647

Number Of Pages


  • 8

Start Page


  • 316

End Page


  • 324

Volume


  • 8

Abstract


  • Natural selection in photosynthesis has engineered tetrapyrrole based, nanometer scale, light harvesting

    and energy capture in light-induced charge separation. By designing and creating nanometer scale

    artificial light harvesting and charge separating proteins, we have the opportunity to reengineer and

    overcome the limitations of natural selection to extend energy capture to new wavelengths and to tailor

    efficient systems that better meet human as opposed to cellular energetic needs. While tetrapyrrole

    cofactor incorporation in natural proteins is complex and often assisted by accessory proteins for

    cofactor transport and insertion, artificial protein functionalization relies on a practical understanding of

    the basic physical chemistry of protein and cofactors that drive nanometer scale self-assembly.

    Patterning and balancing of hydrophobic and hydrophilic tetrapyrrole substituents is critical to avoid

    natural or synthetic porphyrin and chlorin aggregation in aqueous media and speed cofactor partitioning

    into the non-polar core of a man-made water soluble protein designed according to elementary first

    principles of protein folding. This partitioning is followed by site-specific anchoring of tetrapyrroles to

    histidine ligands strategically placed for design control of rates and efficiencies of light energy and

    electron transfer while orienting at least one polar group towards the aqueous phase

Authors


  •   Kodali, Goutham (external author)
  •   Mancini, Joshua A. (external author)
  •   Solomon, Lee A. (external author)
  •   Episova, Tatiana V. (external author)
  •   Roach, Nicholas (external author)
  •   Hobbs, Christopher (external author)
  •   Wagner, Pawel
  •   Mass, Olga (external author)
  •   Aravindu, Kunche (external author)
  •   Barnsley, Jonathan E. (external author)
  •   Gordon, Keith C. (external author)
  •   Officer, David L.
  •   Dutton, P. Leslie (external author)
  •   Moser, Christopher C. (external author)

Publication Date


  • 2017

Citation


  • Kodali, G., Mancini, J. A., Solomon, L. A., Episova, T., Roach, N., Hobbs, C., Wagner, P. W., Mass, O., Aravindu, K., Barnsley, J. E., Gordon, K. C., Officer, D. L., Dutton, P. & Moser, C. C. (2017). Design and engineering of water-soluble light-harvesting protein maquettes. Chemical Science, 8 316-324.

Scopus Eid


  • 2-s2.0-85007150647

Number Of Pages


  • 8

Start Page


  • 316

End Page


  • 324

Volume


  • 8