Skip to main content
placeholder image

Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation

Journal Article


Download full-text (Open Access)

Abstract


  • Enzymatic treatment can effectively degrade recalcitrant micro-pollutants in wastewater. However, its industrial application is constrained by the high cost of the purified enzyme preparations. This work introduces a novel technique to directly immobilize the in-house crude enzyme extracts (from P. ostreatus) onto the functionalized TiO2 nanoparticle surface. Comprehensive investigations were carried out to understand the interactions between complex crude enzyme extracts and the immobilization support. By simple dilution of the crude enzyme extract, the immobilization efficiency can be significantly improved. The resultant biocatalytic nanoparticles had comparable performance to the immobilized purified commercial enzymes. Finally, the micropollutant degradation capability of the biocatalytic nanoparticles was demonstrated with two micro-pollutants, namely, bisphenol-A and carbamazepine, commonly detected in sewage. The efficient laccase extraction and immobilization on biocatalytic nanoparticles show great promise as a cost-effective alternative to conventional wastewater treatment processes for recalcitrant micro-pollutants.

Authors


  •   Ji, Chao (external author)
  •   Nguyen, Luong (external author)
  •   Hou, Jingwei (external author)
  •   Hai, Faisal I.
  •   Chen, Vicki (external author)

Publication Date


  • 2017

Citation


  • Ji, C., Nguyen, L. N., Hou, J., Hai, F. I. & Chen, V. (2017). Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation. Separation and Purification Technology, 178 215-223.

Scopus Eid


  • 2-s2.0-85010790090

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=7455&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/6425

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 215

End Page


  • 223

Volume


  • 178

Place Of Publication


  • United Kingdom

Abstract


  • Enzymatic treatment can effectively degrade recalcitrant micro-pollutants in wastewater. However, its industrial application is constrained by the high cost of the purified enzyme preparations. This work introduces a novel technique to directly immobilize the in-house crude enzyme extracts (from P. ostreatus) onto the functionalized TiO2 nanoparticle surface. Comprehensive investigations were carried out to understand the interactions between complex crude enzyme extracts and the immobilization support. By simple dilution of the crude enzyme extract, the immobilization efficiency can be significantly improved. The resultant biocatalytic nanoparticles had comparable performance to the immobilized purified commercial enzymes. Finally, the micropollutant degradation capability of the biocatalytic nanoparticles was demonstrated with two micro-pollutants, namely, bisphenol-A and carbamazepine, commonly detected in sewage. The efficient laccase extraction and immobilization on biocatalytic nanoparticles show great promise as a cost-effective alternative to conventional wastewater treatment processes for recalcitrant micro-pollutants.

Authors


  •   Ji, Chao (external author)
  •   Nguyen, Luong (external author)
  •   Hou, Jingwei (external author)
  •   Hai, Faisal I.
  •   Chen, Vicki (external author)

Publication Date


  • 2017

Citation


  • Ji, C., Nguyen, L. N., Hou, J., Hai, F. I. & Chen, V. (2017). Direct immobilization of laccase on titania nanoparticles from crude enzyme extracts of P. ostreatus culture for micro-pollutant degradation. Separation and Purification Technology, 178 215-223.

Scopus Eid


  • 2-s2.0-85010790090

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=7455&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/6425

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 215

End Page


  • 223

Volume


  • 178

Place Of Publication


  • United Kingdom