Skip to main content
placeholder image

Watching cellular machinery in action, one molecule at a time

Journal Article


Download full-text (Open Access)

Abstract


  • Single-molecule manipulation and imaging techniques have become important elements of the biologist's toolkit to gain mechanistic insights into cellular processes. By removing ensemble averaging, single-molecule methods provide unique access to the dynamic behavior of biomolecules. Recently, the use of these approaches has expanded to the study of complex multiprotein systems and has enabled detailed characterization of the behavior of individual molecules inside living cells. In this review, we provide an overview of the various force-and fluorescence-based single-molecule methods with applications both in vitro and in vivo, highlighting these advances by describing their applications in studies on cytoskeletal motors and DNA replication. We also discuss how singlemolecule approaches have increased our understanding of the dynamic behavior of complex multiprotein systems. These methods have shown that the behavior of multicomponent protein complexes is highly stochastic and less linear and deterministic than previously thought. Further development of single-molecule tools will help to elucidate the molecular dynamics of these complex systems both inside the cell and in solutions with purified components.

Publication Date


  • 2017

Citation


  • Monachino, E., Spenkelink, L. M. & van Oijen, A. M. (2017). Watching cellular machinery in action, one molecule at a time. The Journal of Cell Biology, 216 (1), 41-51.

Scopus Eid


  • 2-s2.0-85008698390

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2029&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1004

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 41

End Page


  • 51

Volume


  • 216

Issue


  • 1

Place Of Publication


  • United States

Abstract


  • Single-molecule manipulation and imaging techniques have become important elements of the biologist's toolkit to gain mechanistic insights into cellular processes. By removing ensemble averaging, single-molecule methods provide unique access to the dynamic behavior of biomolecules. Recently, the use of these approaches has expanded to the study of complex multiprotein systems and has enabled detailed characterization of the behavior of individual molecules inside living cells. In this review, we provide an overview of the various force-and fluorescence-based single-molecule methods with applications both in vitro and in vivo, highlighting these advances by describing their applications in studies on cytoskeletal motors and DNA replication. We also discuss how singlemolecule approaches have increased our understanding of the dynamic behavior of complex multiprotein systems. These methods have shown that the behavior of multicomponent protein complexes is highly stochastic and less linear and deterministic than previously thought. Further development of single-molecule tools will help to elucidate the molecular dynamics of these complex systems both inside the cell and in solutions with purified components.

Publication Date


  • 2017

Citation


  • Monachino, E., Spenkelink, L. M. & van Oijen, A. M. (2017). Watching cellular machinery in action, one molecule at a time. The Journal of Cell Biology, 216 (1), 41-51.

Scopus Eid


  • 2-s2.0-85008698390

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2029&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/1004

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 41

End Page


  • 51

Volume


  • 216

Issue


  • 1

Place Of Publication


  • United States