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Mechanism of membrane fusion induced by vesicular stomatitis virus G protein

Journal Article


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Abstract


  • The glycoproteins (G proteins) of vesicular stomatitis virus (VSV) and related rhabdoviruses (e.g., rabies virus) mediate both cell attachment and membrane fusion. The reversibility of their fusogenic conformational transitions differentiates them from many other low-pH-induced viral fusion proteins. We report single-virion fusion experiments, using methods developed in previous publications to probe fusion of influenza and West Nile viruses. We show that a three-stage model fits VSV single-particle fusion kinetics: (i) reversible, pH-dependent, G-protein conformational change from the known prefusion conformation to an extended, monomeric intermediate; (ii) reversible trimerization and clustering of the G-protein fusion loops, leading to an extended intermediate that inserts the fusion loops into the target-cell membrane; and (iii) folding back of a cluster of extended trimers into their postfusion conformations, bringing together the viral and cellular membranes. From simulations of the kinetic data, we conclude that the critical number of G-protein trimers required to overcome membrane resistance is 3 to 5, within a contact zone between the virus and the target membrane of 30 to 50 trimers. This sequence of conformational events is similar to those shown to describe fusion by influenza virus hemagglutinin (a "class I" fusogen) and West Nile virus envelope protein ("class II"). Our study of VSV now extends this description to "class III" viral fusion proteins, showing that reversibility of the low-pHinduced transition and architectural differences in the fusion proteins themselves do not change the basic mechanism by which they catalyze membrane fusion.

Authors


  •   Kim, Irene S. (external author)
  •   Jenni, Simon (external author)
  •   Stanifer, Megan (external author)
  •   Roth, Eatai (external author)
  •   Whelan, Sean P. (external author)
  •   van Oijen, Antoine M.
  •   Harrison, Stephen C. (external author)

Publication Date


  • 2017

Citation


  • Kim, I. S., Jenni, S., Stanifer, M. L., Roth, E., Whelan, S. P. J., van Oijen, A. M. & Harrison, S. C. (2017). Mechanism of membrane fusion induced by vesicular stomatitis virus G protein. Proceedings of the National Academy of Sciences of USA, 114 (1), E28-E36.

Scopus Eid


  • 2-s2.0-85007524030

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5531&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4507

Has Global Citation Frequency


Start Page


  • E28

End Page


  • E36

Volume


  • 114

Issue


  • 1

Place Of Publication


  • United States

Abstract


  • The glycoproteins (G proteins) of vesicular stomatitis virus (VSV) and related rhabdoviruses (e.g., rabies virus) mediate both cell attachment and membrane fusion. The reversibility of their fusogenic conformational transitions differentiates them from many other low-pH-induced viral fusion proteins. We report single-virion fusion experiments, using methods developed in previous publications to probe fusion of influenza and West Nile viruses. We show that a three-stage model fits VSV single-particle fusion kinetics: (i) reversible, pH-dependent, G-protein conformational change from the known prefusion conformation to an extended, monomeric intermediate; (ii) reversible trimerization and clustering of the G-protein fusion loops, leading to an extended intermediate that inserts the fusion loops into the target-cell membrane; and (iii) folding back of a cluster of extended trimers into their postfusion conformations, bringing together the viral and cellular membranes. From simulations of the kinetic data, we conclude that the critical number of G-protein trimers required to overcome membrane resistance is 3 to 5, within a contact zone between the virus and the target membrane of 30 to 50 trimers. This sequence of conformational events is similar to those shown to describe fusion by influenza virus hemagglutinin (a "class I" fusogen) and West Nile virus envelope protein ("class II"). Our study of VSV now extends this description to "class III" viral fusion proteins, showing that reversibility of the low-pHinduced transition and architectural differences in the fusion proteins themselves do not change the basic mechanism by which they catalyze membrane fusion.

Authors


  •   Kim, Irene S. (external author)
  •   Jenni, Simon (external author)
  •   Stanifer, Megan (external author)
  •   Roth, Eatai (external author)
  •   Whelan, Sean P. (external author)
  •   van Oijen, Antoine M.
  •   Harrison, Stephen C. (external author)

Publication Date


  • 2017

Citation


  • Kim, I. S., Jenni, S., Stanifer, M. L., Roth, E., Whelan, S. P. J., van Oijen, A. M. & Harrison, S. C. (2017). Mechanism of membrane fusion induced by vesicular stomatitis virus G protein. Proceedings of the National Academy of Sciences of USA, 114 (1), E28-E36.

Scopus Eid


  • 2-s2.0-85007524030

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5531&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4507

Has Global Citation Frequency


Start Page


  • E28

End Page


  • E36

Volume


  • 114

Issue


  • 1

Place Of Publication


  • United States