Superconducting wires are widely used in fabricating magnetic coils in fusion reactors. In consideration of the stability of 11B against neutron irradiation and lower induced radio-activation properties, MgB2 superconductor with 11B serving as boron source is an alternative candidate to be used in fusion reactor with severe irradiation environment. In present work, a batch of monofilament isotopic Mg11B2 wires with amorphous 11B powder as precursor were fabricated using powder-in-tube (PIT) process at different sintering temperature, and the evolution of their microstructure and corresponding superconducting properties was systemically investigated. Accordingly, the best transport critical current density (Jc) = 2 × 104 A/cm2 was obtained at 4.2 K and 5 T, which is even comparable to multi-filament Mg11B2 isotope wires reported in other work. Surprisingly, transport Jc vanished in our wire which was heat-treated at excessively high temperature (800 °C). Combined with microstructure observation, it was found that lots of big interconnected microcracks and voids that can isolate the MgB2 grains formed in this whole sample, resulting in significant deterioration in inter-grain connectivity. The results can be a constructive guide in fabricating Mg11B2 wires to be used as magnet coils in fusion reactor systems such as ITER-type tokamak magnet.