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P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes

Journal Article


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Abstract


  • AIM

    To determine if activation of the ATP-gated P2X7 receptor

    channel induces phosphatidylserine (PS) exposure in

    erythrocytes from multiple dog breeds.

    METHODS

    Peripheral blood was collected from 25 dogs representing

    13 pedigrees and seven crossbreeds. ATP-induced PS

    exposure on canine erythrocytes in vitro was assessed

    using a flow cytometric Annexin V binding assay.

    RESULTS

    ATP induced PS exposure in erythrocytes from all dogs

    studied. ATP caused PS exposure in a concentrationdependent

    manner with an EC50 value of 395 μmol/L.

    The non-P2X7 agonists, ADP or AMP, did not cause PS

    exposure. The P2X7 antagonist, AZ10606120, but not

    the P2X1 antagonist, NF449, blocked ATP-induced PS

    exposure.

    CONCLUSION

    The results indicate that ATP induces PS exposure in

    erythrocytes from various dog breeds and that this

    process is mediated by P2X7 activation.

UOW Authors


Publication Date


  • 2016

Citation


  • Faulks, M., Kuit, T. A., Sophocleous, R. A., Curtis, B. L., Curtis, S. J., Jurak, L. M. & Sluyter, R. (2016). P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes. World Journal of Hematology, 5 (4), 88-93.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5578&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4554

Number Of Pages


  • 5

Start Page


  • 88

End Page


  • 93

Volume


  • 5

Issue


  • 4

Place Of Publication


  • United States

Abstract


  • AIM

    To determine if activation of the ATP-gated P2X7 receptor

    channel induces phosphatidylserine (PS) exposure in

    erythrocytes from multiple dog breeds.

    METHODS

    Peripheral blood was collected from 25 dogs representing

    13 pedigrees and seven crossbreeds. ATP-induced PS

    exposure on canine erythrocytes in vitro was assessed

    using a flow cytometric Annexin V binding assay.

    RESULTS

    ATP induced PS exposure in erythrocytes from all dogs

    studied. ATP caused PS exposure in a concentrationdependent

    manner with an EC50 value of 395 μmol/L.

    The non-P2X7 agonists, ADP or AMP, did not cause PS

    exposure. The P2X7 antagonist, AZ10606120, but not

    the P2X1 antagonist, NF449, blocked ATP-induced PS

    exposure.

    CONCLUSION

    The results indicate that ATP induces PS exposure in

    erythrocytes from various dog breeds and that this

    process is mediated by P2X7 activation.

UOW Authors


Publication Date


  • 2016

Citation


  • Faulks, M., Kuit, T. A., Sophocleous, R. A., Curtis, B. L., Curtis, S. J., Jurak, L. M. & Sluyter, R. (2016). P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes. World Journal of Hematology, 5 (4), 88-93.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5578&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4554

Number Of Pages


  • 5

Start Page


  • 88

End Page


  • 93

Volume


  • 5

Issue


  • 4

Place Of Publication


  • United States