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P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes

Journal Article


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Abstract


  • AIM

    To determine if activation of the ATP-gated P2X7 receptor

    channel induces phosphatidylserine (PS) exposure in

    erythrocytes from multiple dog breeds.

    METHODS

    Peripheral blood was collected from 25 dogs representing

    13 pedigrees and seven crossbreeds. ATP-induced PS

    exposure on canine erythrocytes in vitro was assessed

    using a flow cytometric Annexin V binding assay.

    RESULTS

    ATP induced PS exposure in erythrocytes from all dogs

    studied. ATP caused PS exposure in a concentrationdependent

    manner with an EC50 value of 395 μmol/L.

    The non-P2X7 agonists, ADP or AMP, did not cause PS

    exposure. The P2X7 antagonist, AZ10606120, but not

    the P2X1 antagonist, NF449, blocked ATP-induced PS

    exposure.

    CONCLUSION

    The results indicate that ATP induces PS exposure in

    erythrocytes from various dog breeds and that this

    process is mediated by P2X7 activation.

Authors


  •   Faulks, Megan (external author)
  •   Kuit, Tracey A.
  •   Sophocleous, Reece (external author)
  •   Curtis, Belinda L. (external author)
  •   Curtis, Stephen J. (external author)
  •   Jurak, Lisa M. (external author)
  •   Sluyter, Ronald

Publication Date


  • 2016

Citation


  • Faulks, M., Kuit, T. A., Sophocleous, R. A., Curtis, B. L., Curtis, S. J., Jurak, L. M. & Sluyter, R. (2016). P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes. World Journal of Hematology, 5 (4), 88-93.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5578&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4554

Number Of Pages


  • 5

Start Page


  • 88

End Page


  • 93

Volume


  • 5

Issue


  • 4

Place Of Publication


  • United States

Abstract


  • AIM

    To determine if activation of the ATP-gated P2X7 receptor

    channel induces phosphatidylserine (PS) exposure in

    erythrocytes from multiple dog breeds.

    METHODS

    Peripheral blood was collected from 25 dogs representing

    13 pedigrees and seven crossbreeds. ATP-induced PS

    exposure on canine erythrocytes in vitro was assessed

    using a flow cytometric Annexin V binding assay.

    RESULTS

    ATP induced PS exposure in erythrocytes from all dogs

    studied. ATP caused PS exposure in a concentrationdependent

    manner with an EC50 value of 395 μmol/L.

    The non-P2X7 agonists, ADP or AMP, did not cause PS

    exposure. The P2X7 antagonist, AZ10606120, but not

    the P2X1 antagonist, NF449, blocked ATP-induced PS

    exposure.

    CONCLUSION

    The results indicate that ATP induces PS exposure in

    erythrocytes from various dog breeds and that this

    process is mediated by P2X7 activation.

Authors


  •   Faulks, Megan (external author)
  •   Kuit, Tracey A.
  •   Sophocleous, Reece (external author)
  •   Curtis, Belinda L. (external author)
  •   Curtis, Stephen J. (external author)
  •   Jurak, Lisa M. (external author)
  •   Sluyter, Ronald

Publication Date


  • 2016

Citation


  • Faulks, M., Kuit, T. A., Sophocleous, R. A., Curtis, B. L., Curtis, S. J., Jurak, L. M. & Sluyter, R. (2016). P2X7 receptor activation causes phosphatidylserine exposure in canine erythrocytes. World Journal of Hematology, 5 (4), 88-93.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5578&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4554

Number Of Pages


  • 5

Start Page


  • 88

End Page


  • 93

Volume


  • 5

Issue


  • 4

Place Of Publication


  • United States