To determine if activation of the ATP-gated P2X7 receptor
channel induces phosphatidylserine (PS) exposure in
erythrocytes from multiple dog breeds.
Peripheral blood was collected from 25 dogs representing
13 pedigrees and seven crossbreeds. ATP-induced PS
exposure on canine erythrocytes in vitro was assessed
using a flow cytometric Annexin V binding assay.
ATP induced PS exposure in erythrocytes from all dogs
studied. ATP caused PS exposure in a concentrationdependent
manner with an EC50 value of 395 μmol/L.
The non-P2X7 agonists, ADP or AMP, did not cause PS
exposure. The P2X7 antagonist, AZ10606120, but not
the P2X1 antagonist, NF449, blocked ATP-induced PS
The results indicate that ATP induces PS exposure in
erythrocytes from various dog breeds and that this
process is mediated by P2X7 activation.