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Rapid flow cytometric measurement of protein inclusions and nuclear trafficking

Journal Article


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Abstract


  • Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.

Publication Date


  • 2016

Citation


  • Whiten, D. R., San Gil, R., McAlary, L., Yerbury, J. J., Ecroyd, H. & Wilson, M. R. (2016). Rapid flow cytometric measurement of protein inclusions and nuclear trafficking. Scientific Reports, 6 31138-1-31138-9.

Scopus Eid


  • 2-s2.0-84982299471

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1925&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/901

Has Global Citation Frequency


Start Page


  • 31138-1

End Page


  • 31138-9

Volume


  • 6

Place Of Publication


  • United Kingdom

Abstract


  • Proteinaceous cytoplasmic inclusions are an indicator of dysfunction in normal cellular proteostasis and a hallmark of many neurodegenerative diseases. We describe a simple and rapid new flow cytometry-based method to enumerate, characterise and, if desired, physically recover protein inclusions from cells. This technique can analyse and resolve a broad variety of inclusions differing in both size and protein composition, making it applicable to essentially any model of intracellular protein aggregation. The method also allows rapid quantification of the nuclear trafficking of fluorescently labelled molecules.

Publication Date


  • 2016

Citation


  • Whiten, D. R., San Gil, R., McAlary, L., Yerbury, J. J., Ecroyd, H. & Wilson, M. R. (2016). Rapid flow cytometric measurement of protein inclusions and nuclear trafficking. Scientific Reports, 6 31138-1-31138-9.

Scopus Eid


  • 2-s2.0-84982299471

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1925&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/901

Has Global Citation Frequency


Start Page


  • 31138-1

End Page


  • 31138-9

Volume


  • 6

Place Of Publication


  • United Kingdom