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5′-substituted amiloride derivatives as allosteric modulators binding in the sodium ion pocket of the adenosine A2A receptor

Journal Article


Abstract


  • The sodium ion site is an allosteric site conserved among many G protein-coupled receptors (GPCRs). Amiloride 1 and 5-(N,N-hexamethylene)amiloride 2 (HMA) supposedly bind in this sodium ion site and can influence orthosteric ligand binding. The availability of a high-resolution X-ray crystal structure of the human adenosine A2A receptor (hA2AAR), in which the allosteric sodium ion site was elucidated, makes it an appropriate model receptor for investigating the allosteric site. In this study, we report the synthesis and evaluation of novel 5′-substituted amiloride derivatives as hA2AAR allosteric antagonists. The potency of the amiloride derivatives was assessed by their ability to displace orthosteric radioligand [3H]4-(2-((7-amino-2-(furan-2-yl)-[1,2,4]triazolo[1,5-a]-[1,3,5]triazin-5-yl)amino)ethyl)phenol ([3H]ZM-241,385) from both the wild-type and sodium ion site W246A mutant hA2AAR. 4-Ethoxyphenethyl-substituted amiloride 12l was found to be more potent than both amiloride and HMA, and the shift in potency between the wild-type and mutated receptor confirmed its likely binding to the sodium ion site.

Authors


  •   Massink, Arnault (external author)
  •   Louvel, Julien (external author)
  •   Adlere, Ilze (external author)
  •   van Veen, Corine (external author)
  •   Huisman, Berend (external author)
  •   Dijksteel, Gabrielle S. (external author)
  •   Guo, Dong (external author)
  •   Lenselink, Eelke B. (external author)
  •   Buckley, Benjamin J.
  •   Matthews, Hayden (external author)
  •   Ranson, Marie
  •   Kelso, Michael J.
  •   Ijzerman, Adriaan P. (external author)

Publication Date


  • 2016

Citation


  • Massink, A., Louvel, J., Adlere, I., van Veen, C., Huisman, B. J. H., Dijksteel, G. S., Guo, D., Lenselink, E. B., Buckley, B. J., Matthews, H., Ranson, M., Kelso, M. & Ijzerman, A. P. (2016). 5′-substituted amiloride derivatives as allosteric modulators binding in the sodium ion pocket of the adenosine A2A receptor. Journal of Medicinal Chemistry, 59 (10), 4769-4777.

Scopus Eid


  • 2-s2.0-84971622003

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4004

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 4769

End Page


  • 4777

Volume


  • 59

Issue


  • 10

Place Of Publication


  • United States

Abstract


  • The sodium ion site is an allosteric site conserved among many G protein-coupled receptors (GPCRs). Amiloride 1 and 5-(N,N-hexamethylene)amiloride 2 (HMA) supposedly bind in this sodium ion site and can influence orthosteric ligand binding. The availability of a high-resolution X-ray crystal structure of the human adenosine A2A receptor (hA2AAR), in which the allosteric sodium ion site was elucidated, makes it an appropriate model receptor for investigating the allosteric site. In this study, we report the synthesis and evaluation of novel 5′-substituted amiloride derivatives as hA2AAR allosteric antagonists. The potency of the amiloride derivatives was assessed by their ability to displace orthosteric radioligand [3H]4-(2-((7-amino-2-(furan-2-yl)-[1,2,4]triazolo[1,5-a]-[1,3,5]triazin-5-yl)amino)ethyl)phenol ([3H]ZM-241,385) from both the wild-type and sodium ion site W246A mutant hA2AAR. 4-Ethoxyphenethyl-substituted amiloride 12l was found to be more potent than both amiloride and HMA, and the shift in potency between the wild-type and mutated receptor confirmed its likely binding to the sodium ion site.

Authors


  •   Massink, Arnault (external author)
  •   Louvel, Julien (external author)
  •   Adlere, Ilze (external author)
  •   van Veen, Corine (external author)
  •   Huisman, Berend (external author)
  •   Dijksteel, Gabrielle S. (external author)
  •   Guo, Dong (external author)
  •   Lenselink, Eelke B. (external author)
  •   Buckley, Benjamin J.
  •   Matthews, Hayden (external author)
  •   Ranson, Marie
  •   Kelso, Michael J.
  •   Ijzerman, Adriaan P. (external author)

Publication Date


  • 2016

Citation


  • Massink, A., Louvel, J., Adlere, I., van Veen, C., Huisman, B. J. H., Dijksteel, G. S., Guo, D., Lenselink, E. B., Buckley, B. J., Matthews, H., Ranson, M., Kelso, M. & Ijzerman, A. P. (2016). 5′-substituted amiloride derivatives as allosteric modulators binding in the sodium ion pocket of the adenosine A2A receptor. Journal of Medicinal Chemistry, 59 (10), 4769-4777.

Scopus Eid


  • 2-s2.0-84971622003

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/4004

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 4769

End Page


  • 4777

Volume


  • 59

Issue


  • 10

Place Of Publication


  • United States