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High throughput cell-free extraction of plasma by an integrated microfluidic device combining inertial microfluidics and membrane

Conference Paper


Abstract


  • Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction.

UOW Authors


  •   Yuan, Dan (external author)
  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Li, Weihua

Publication Date


  • 2016

Citation


  • Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N. & Li, W. (2016). High throughput cell-free extraction of plasma by an integrated microfluidic device combining inertial microfluidics and membrane. ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer: Volume 1 (pp. V001T01A012-1-V001T01A012-6). United States: ASME.

Scopus Eid


  • 2-s2.0-84968813474

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/8

Has Global Citation Frequency


Start Page


  • V001T01A012-1

End Page


  • V001T01A012-6

Place Of Publication


  • United States

Abstract


  • Plasma is a host of various analytes such as proteins, metabolites, circulating nucleic acids (CNAs), pathogens. The key process of plasma extraction is to eliminate the contamination from blood cells. Conventional methods, such as centrifugation and membrane filtration, are generally lab-intensive, time consuming and even dangerous. In this study, we report an integrated microfluidic device that combines inertial microfluidics and membrane filter. The integrated microfluidic device was evaluated by the diluted (x1/10, x1/20) whole blood, and the quality of the extracted blood plasma was tested. It was found that quality of extracted blood plasma from integrated device was equivalent to that obtained by the centrifugation. This study demonstrates a significant progress towards the practical application of inertial microfluidics with membrane filter for high-throughput and high efficient blood plasma extraction.

UOW Authors


  •   Yuan, Dan (external author)
  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)
  •   Li, Weihua

Publication Date


  • 2016

Citation


  • Zhang, J., Yan, S., Yuan, D., Alici, G., Nguyen, N. & Li, W. (2016). High throughput cell-free extraction of plasma by an integrated microfluidic device combining inertial microfluidics and membrane. ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer: Volume 1 (pp. V001T01A012-1-V001T01A012-6). United States: ASME.

Scopus Eid


  • 2-s2.0-84968813474

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers1/8

Has Global Citation Frequency


Start Page


  • V001T01A012-1

End Page


  • V001T01A012-6

Place Of Publication


  • United States