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A tool for alignment and averaging of sparse fluorescence signals in rod-shaped bacteria

Journal Article


Abstract


  • Fluorescence microscopy studies have shown that many proteins localize to highly specific subregions within bacterial cells. Analyzing the spatial distribution of low-abundance proteins within cells is highly challenging because information obtained from multiple cells needs to be combined to provide well-defined maps of protein locations. We present (to our knowledge) a novel tool for fast, automated, and user-impartial analysis of fluorescent protein distribution across the short axis of rod-shaped bacteria. To demonstrate the strength of our approach in extracting spatial distributions and visualizing dynamic intracellular processes, we analyzed sparse fluorescence signals from single-molecule time-lapse images of individual Escherichia coli cells. In principle, our tool can be used to provide information on the distribution of signal intensity across the short axis of any rod-shaped object.

Publication Date


  • 2016

Citation


  • Goudsmits, J. M. H., van Oijen, A. M. & Robinson, A. (2016). A tool for alignment and averaging of sparse fluorescence signals in rod-shaped bacteria. Biophysical Journal, 110 (8), 1708-1715.

Scopus Eid


  • 2-s2.0-84964688033

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/3786

Number Of Pages


  • 7

Start Page


  • 1708

End Page


  • 1715

Volume


  • 110

Issue


  • 8

Place Of Publication


  • United States

Abstract


  • Fluorescence microscopy studies have shown that many proteins localize to highly specific subregions within bacterial cells. Analyzing the spatial distribution of low-abundance proteins within cells is highly challenging because information obtained from multiple cells needs to be combined to provide well-defined maps of protein locations. We present (to our knowledge) a novel tool for fast, automated, and user-impartial analysis of fluorescent protein distribution across the short axis of rod-shaped bacteria. To demonstrate the strength of our approach in extracting spatial distributions and visualizing dynamic intracellular processes, we analyzed sparse fluorescence signals from single-molecule time-lapse images of individual Escherichia coli cells. In principle, our tool can be used to provide information on the distribution of signal intensity across the short axis of any rod-shaped object.

Publication Date


  • 2016

Citation


  • Goudsmits, J. M. H., van Oijen, A. M. & Robinson, A. (2016). A tool for alignment and averaging of sparse fluorescence signals in rod-shaped bacteria. Biophysical Journal, 110 (8), 1708-1715.

Scopus Eid


  • 2-s2.0-84964688033

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/3786

Number Of Pages


  • 7

Start Page


  • 1708

End Page


  • 1715

Volume


  • 110

Issue


  • 8

Place Of Publication


  • United States