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Functional 3D neural mini-tissues from printed gel-based bioink and human neural stem cells

Journal Article


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Abstract


  • Direct-write printing of stem cells within biomaterials presents an opportunity to engineer tissue for in vitro modeling and regenerative medicine. Here, a first example of constructing neural tissue by printing human neural stem cells that are differentiated in situ to functional neurons and supporting neuroglia is reported. The supporting biomaterial incorporates a novel clinically relevant polysaccharide-based bioink comprising alginate, carboxymethyl-chitosan, and agarose. The printed bioink rapidly gels by stable cross-linking to form a porous 3D scaffold encapsulating stem cells for in situ expansion and differentiation. Differentiated neurons form synaptic contacts, establish networks, are spontaneously active, show a bicuculline-induced increased calcium response, and are predominantly gamma-aminobutyric acid expressing. The 3D tissues will facilitate investigation of human neural development, function, and disease, and may be adaptable for engineering other 3D tissues from different stem cell types.

Publication Date


  • 2016

Citation


  • Gu, Q., Tomaskovic-Crook, E., Lozano, R., Chen, Y., Kapsa, R. M. I., Zhou, Q., Wallace, G. G. & Crook, J. M. (2016). Functional 3D neural mini-tissues from printed gel-based bioink and human neural stem cells. Advanced Healthcare Materials, 5 (12), 1429-1438.

Scopus Eid


  • 2-s2.0-84976590594

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3160&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2139

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 1429

End Page


  • 1438

Volume


  • 5

Issue


  • 12

Place Of Publication


  • Germany

Abstract


  • Direct-write printing of stem cells within biomaterials presents an opportunity to engineer tissue for in vitro modeling and regenerative medicine. Here, a first example of constructing neural tissue by printing human neural stem cells that are differentiated in situ to functional neurons and supporting neuroglia is reported. The supporting biomaterial incorporates a novel clinically relevant polysaccharide-based bioink comprising alginate, carboxymethyl-chitosan, and agarose. The printed bioink rapidly gels by stable cross-linking to form a porous 3D scaffold encapsulating stem cells for in situ expansion and differentiation. Differentiated neurons form synaptic contacts, establish networks, are spontaneously active, show a bicuculline-induced increased calcium response, and are predominantly gamma-aminobutyric acid expressing. The 3D tissues will facilitate investigation of human neural development, function, and disease, and may be adaptable for engineering other 3D tissues from different stem cell types.

Publication Date


  • 2016

Citation


  • Gu, Q., Tomaskovic-Crook, E., Lozano, R., Chen, Y., Kapsa, R. M. I., Zhou, Q., Wallace, G. G. & Crook, J. M. (2016). Functional 3D neural mini-tissues from printed gel-based bioink and human neural stem cells. Advanced Healthcare Materials, 5 (12), 1429-1438.

Scopus Eid


  • 2-s2.0-84976590594

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3160&context=aiimpapers

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2139

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 1429

End Page


  • 1438

Volume


  • 5

Issue


  • 12

Place Of Publication


  • Germany