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Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr−/− mice

Journal Article


Abstract


  • Although it is recognized that lipids and membrane organization in T cells affect signaling and T cell activation, to what extent dietary lipids alter T cell responsiveness in the absence of obesity and inflammation is not known. In this study, we fed low-density lipoprotein receptor knockout mice a Western high-fat diet for 1 or 9 wk and examined T cell responses in vivo along with T cell lipid composition, membrane order, and activation ex vivo. Our data showed that high levels of circulating lipids for a prolonged period elevated CD4+ and CD8+ T cell proliferation and resulted in an increased proportion of CD4+ central-memory T cells within the draining lymph nodes following induction of contact hypersensitivity. In addition, the 9-wk Western high-fat diet elevated the total phospholipid content and monounsaturated fatty acid level, but decreased saturated phosphatidylcholine and sphingomyelin within the T cells. The altered lipid composition in the circulation, and of T cells, was also reflected by enhanced membrane order at the activation site of ex vivo activated T cells that corresponded to increased IL-2 mRNA levels. In conclusion, dietary lipids can modulate T cell lipid composition and responses in lipoprotein receptor knockout mice even in the absence of excess weight gain and a proinflammatory environment.

UOW Authors


  •   Pollock, Abigail H. (external author)
  •   Tedla, Nicodemus (external author)
  •   Hancock, Sarah
  •   Cornely, Rhea (external author)
  •   Mitchell, Todd
  •   Yang, Zhengmin (external author)
  •   Kockx, Maaike (external author)
  •   Parton, Robert G. (external author)
  •   Rossy, Jérémie (external author)
  •   Gaus, Katharina (external author)

Publication Date


  • 2016

Citation


  • Pollock, A. H., Tedla, N., Hancock, S. E., Cornely, R., Mitchell, T. W., Yang, Z., Kockx, M., Parton, R. G., Rossy, J. & Gaus, K. (2016). Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr−/− mice. Journal of Immunology, 196 (10), 3993-4002.

Scopus Eid


  • 2-s2.0-84975064369

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/838

Number Of Pages


  • 9

Start Page


  • 3993

End Page


  • 4002

Volume


  • 196

Issue


  • 10

Abstract


  • Although it is recognized that lipids and membrane organization in T cells affect signaling and T cell activation, to what extent dietary lipids alter T cell responsiveness in the absence of obesity and inflammation is not known. In this study, we fed low-density lipoprotein receptor knockout mice a Western high-fat diet for 1 or 9 wk and examined T cell responses in vivo along with T cell lipid composition, membrane order, and activation ex vivo. Our data showed that high levels of circulating lipids for a prolonged period elevated CD4+ and CD8+ T cell proliferation and resulted in an increased proportion of CD4+ central-memory T cells within the draining lymph nodes following induction of contact hypersensitivity. In addition, the 9-wk Western high-fat diet elevated the total phospholipid content and monounsaturated fatty acid level, but decreased saturated phosphatidylcholine and sphingomyelin within the T cells. The altered lipid composition in the circulation, and of T cells, was also reflected by enhanced membrane order at the activation site of ex vivo activated T cells that corresponded to increased IL-2 mRNA levels. In conclusion, dietary lipids can modulate T cell lipid composition and responses in lipoprotein receptor knockout mice even in the absence of excess weight gain and a proinflammatory environment.

UOW Authors


  •   Pollock, Abigail H. (external author)
  •   Tedla, Nicodemus (external author)
  •   Hancock, Sarah
  •   Cornely, Rhea (external author)
  •   Mitchell, Todd
  •   Yang, Zhengmin (external author)
  •   Kockx, Maaike (external author)
  •   Parton, Robert G. (external author)
  •   Rossy, Jérémie (external author)
  •   Gaus, Katharina (external author)

Publication Date


  • 2016

Citation


  • Pollock, A. H., Tedla, N., Hancock, S. E., Cornely, R., Mitchell, T. W., Yang, Z., Kockx, M., Parton, R. G., Rossy, J. & Gaus, K. (2016). Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr−/− mice. Journal of Immunology, 196 (10), 3993-4002.

Scopus Eid


  • 2-s2.0-84975064369

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/838

Number Of Pages


  • 9

Start Page


  • 3993

End Page


  • 4002

Volume


  • 196

Issue


  • 10