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Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus

Journal Article


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Abstract


  • In tissue engineering of cartilage, polymeric scaffolds

    are implanted in the damaged tissue and subjected to

    repeated compression loading cycles. The possibility of failure

    due to mechanical fatigue has not been properly

    addressed in these scaffolds. Nevertheless, the macroporous

    scaffold is susceptible to failure after repeated loading–

    unloading cycles. This is related to inherent discontinuities in

    the material due to the micropore structure of the macropore

    walls that act as stress concentration points. In this

    work, chondrogenic precursor cells have been seeded in

    poly-e-caprolactone (PCL) scaffolds with fibrin and some were

    submitted to free swelling culture and others to cyclic loading

    in a bioreactor. After cell culture, all the samples were

    analyzed for fatigue behavior under repeated loading–unloading

    cycles. Moreover, some components of the extracellular

    matrix (ECM) were identified. No differences were observed

    between samples undergoing free swelling or bioreactor

    loading conditions, neither respect to matrix components nor

    to mechanical performance to fatigue. The ECM did not

    achieve the desired preponderance of collagen type II over

    collagen type I which is considered the main characteristic of

    hyaline cartilage ECM. However, prediction in PCL with ECM

    constructs was possible up to 600 cycles, an enhanced performance

    when compared to previous works. PCL after cell

    culture presents an improved fatigue resistance, despite the

    fact that the measured elastic modulus at the first cycle was

    similar to PCL with poly(vinyl alcohol) samples. This finding

    suggests that fatigue analysis in tissue engineering constructs

    can provide additional information missed with traditional

    mechanical measurements.

UOW Authors


  •   Panadero, J A. (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Silva, Sonia C.M.. (external author)
  •   Ribeiro, Clarisse (external author)
  •   Correia, Vítor (external author)
  •   Gama, F M. (external author)
  •   Gomez Ribelles, J L. (external author)
  •   Lanceros-Méndez, Senentxu (external author)

Publication Date


  • 2016

Citation


  • Panadero, J. Alberto., Sencadas, V., Silva, S. C.M.., Ribeiro, C., Correia, V., Gama, F. M., Gomez Ribelles, J. Luis. & Lanceros-Méndez, S. (2016). Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 104 (2), 330-338.

Scopus Eid


  • 2-s2.0-84954078144

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6277&context=eispapers

Number Of Pages


  • 8

Start Page


  • 330

End Page


  • 338

Volume


  • 104

Issue


  • 2

Abstract


  • In tissue engineering of cartilage, polymeric scaffolds

    are implanted in the damaged tissue and subjected to

    repeated compression loading cycles. The possibility of failure

    due to mechanical fatigue has not been properly

    addressed in these scaffolds. Nevertheless, the macroporous

    scaffold is susceptible to failure after repeated loading–

    unloading cycles. This is related to inherent discontinuities in

    the material due to the micropore structure of the macropore

    walls that act as stress concentration points. In this

    work, chondrogenic precursor cells have been seeded in

    poly-e-caprolactone (PCL) scaffolds with fibrin and some were

    submitted to free swelling culture and others to cyclic loading

    in a bioreactor. After cell culture, all the samples were

    analyzed for fatigue behavior under repeated loading–unloading

    cycles. Moreover, some components of the extracellular

    matrix (ECM) were identified. No differences were observed

    between samples undergoing free swelling or bioreactor

    loading conditions, neither respect to matrix components nor

    to mechanical performance to fatigue. The ECM did not

    achieve the desired preponderance of collagen type II over

    collagen type I which is considered the main characteristic of

    hyaline cartilage ECM. However, prediction in PCL with ECM

    constructs was possible up to 600 cycles, an enhanced performance

    when compared to previous works. PCL after cell

    culture presents an improved fatigue resistance, despite the

    fact that the measured elastic modulus at the first cycle was

    similar to PCL with poly(vinyl alcohol) samples. This finding

    suggests that fatigue analysis in tissue engineering constructs

    can provide additional information missed with traditional

    mechanical measurements.

UOW Authors


  •   Panadero, J A. (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Silva, Sonia C.M.. (external author)
  •   Ribeiro, Clarisse (external author)
  •   Correia, Vítor (external author)
  •   Gama, F M. (external author)
  •   Gomez Ribelles, J L. (external author)
  •   Lanceros-Méndez, Senentxu (external author)

Publication Date


  • 2016

Citation


  • Panadero, J. Alberto., Sencadas, V., Silva, S. C.M.., Ribeiro, C., Correia, V., Gama, F. M., Gomez Ribelles, J. Luis. & Lanceros-Méndez, S. (2016). Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 104 (2), 330-338.

Scopus Eid


  • 2-s2.0-84954078144

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6277&context=eispapers

Number Of Pages


  • 8

Start Page


  • 330

End Page


  • 338

Volume


  • 104

Issue


  • 2