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Bardoxolone methyl prevents high-fat diet-induced colon inflammation in mice

Journal Article


Abstract


  • Obesity induces chronic, low-grade inflammation, which increases the risk of colon cancer. We investigated the preventive effects of Bardoxolone methyl (BARD) on high-fat diet (HFD)-induced inflammation in a mouse colon. Male C57BL/6J mice (n=7) were fed a HFD (HFD group), HFD plus BARD (10 mg/kg) in drinking water (HFD/BARD group), or normal laboratory chow diet (LFD group) for 21 weeks. In HFD mice, BARD reduced colon thickness and decreased colon weight per length. This was associated with an increase in colon crypt depth and the number of goblet cells per crypt. BARD reduced the expression of F4/80 and CD11c but increased CD206 and IL-10, indicating an anti-inflammatory effect. BARD prevented an increase of the intracellular pro-inflammatory biomarkers (NF-қB, p NF-қB, IL-6, TNF-α) and cell proliferation markers (Cox2 and Ki67). BARD prevented fat deposition in the colon wall and prevented microbial population changes. Overall, we report the preventive effects of BARD on colon inflammation in HFD-fed mice through its regulation of macrophages, NF-қB, cytokines, Cox2 and Ki67, fat deposition and microflora.

Authors


  •   Dinh, Chi H. L. (external author)
  •   Yu, Yinghua
  •   Szabo, Alexander M. (external author)
  •   Zhang, Qingsheng (external author)
  •   Zhang, Peng (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2016

Citation


  • Dinh, C. H. L., Yu, Y., Szabo, A., Zhang, Q., Zhang, P. & Huang, X. (2016). Bardoxolone methyl prevents high-fat diet-induced colon inflammation in mice. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 64 (4), 237-255.

Scopus Eid


  • 2-s2.0-84962009896

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/774

Has Global Citation Frequency


Number Of Pages


  • 18

Start Page


  • 237

End Page


  • 255

Volume


  • 64

Issue


  • 4

Place Of Publication


  • United States

Abstract


  • Obesity induces chronic, low-grade inflammation, which increases the risk of colon cancer. We investigated the preventive effects of Bardoxolone methyl (BARD) on high-fat diet (HFD)-induced inflammation in a mouse colon. Male C57BL/6J mice (n=7) were fed a HFD (HFD group), HFD plus BARD (10 mg/kg) in drinking water (HFD/BARD group), or normal laboratory chow diet (LFD group) for 21 weeks. In HFD mice, BARD reduced colon thickness and decreased colon weight per length. This was associated with an increase in colon crypt depth and the number of goblet cells per crypt. BARD reduced the expression of F4/80 and CD11c but increased CD206 and IL-10, indicating an anti-inflammatory effect. BARD prevented an increase of the intracellular pro-inflammatory biomarkers (NF-қB, p NF-қB, IL-6, TNF-α) and cell proliferation markers (Cox2 and Ki67). BARD prevented fat deposition in the colon wall and prevented microbial population changes. Overall, we report the preventive effects of BARD on colon inflammation in HFD-fed mice through its regulation of macrophages, NF-қB, cytokines, Cox2 and Ki67, fat deposition and microflora.

Authors


  •   Dinh, Chi H. L. (external author)
  •   Yu, Yinghua
  •   Szabo, Alexander M. (external author)
  •   Zhang, Qingsheng (external author)
  •   Zhang, Peng (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2016

Citation


  • Dinh, C. H. L., Yu, Y., Szabo, A., Zhang, Q., Zhang, P. & Huang, X. (2016). Bardoxolone methyl prevents high-fat diet-induced colon inflammation in mice. Journal of Histochemistry and Cytochemistry: imaging the spectrum of cell biology, 64 (4), 237-255.

Scopus Eid


  • 2-s2.0-84962009896

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/774

Has Global Citation Frequency


Number Of Pages


  • 18

Start Page


  • 237

End Page


  • 255

Volume


  • 64

Issue


  • 4

Place Of Publication


  • United States