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3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography

Journal Article


Abstract


  • The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. × 600 mm long) was produced within a 5 × 30 × 30 mm titanium alloy (Ti-6Al-4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 69-76 (for 6-8 proteins respectively) were observed during isothermal separation of proteins at 44 °C which were further increased to 73-77 using a thermal step gradient with programmed temperature from 60 °C to 35 °C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of E.coli was also achieved in less than 35 min with ca. 40 distinguishable peaks at 210 nm.

Authors


  •   Gupta, Vipul (external author)
  •   Talebi, Mohammad (external author)
  •   Deverell, Jeremy (external author)
  •   Sandron, Sara (external author)
  •   Nesterenko, Pavel N. (external author)
  •   Heery, Brendan (external author)
  •   Thompson, Fletcher W. (external author)
  •   Beirne, Stephen T.
  •   Wallace, Gordon G.
  •   Paull, Brett (external author)

Publication Date


  • 2016

Citation


  • Gupta, V., Talebi, M., Deverell, J., Sandron, S., Nesterenko, P. N., Heery, B., Thompson, F., Beirne, S., Wallace, G. G. & Paull, B. (2016). 3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography. Analytica Chimica Acta, 910 84-94.

Scopus Eid


  • 2-s2.0-84955579929

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2040

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 84

End Page


  • 94

Volume


  • 910

Place Of Publication


  • Netherlands

Abstract


  • The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. × 600 mm long) was produced within a 5 × 30 × 30 mm titanium alloy (Ti-6Al-4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 69-76 (for 6-8 proteins respectively) were observed during isothermal separation of proteins at 44 °C which were further increased to 73-77 using a thermal step gradient with programmed temperature from 60 °C to 35 °C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of E.coli was also achieved in less than 35 min with ca. 40 distinguishable peaks at 210 nm.

Authors


  •   Gupta, Vipul (external author)
  •   Talebi, Mohammad (external author)
  •   Deverell, Jeremy (external author)
  •   Sandron, Sara (external author)
  •   Nesterenko, Pavel N. (external author)
  •   Heery, Brendan (external author)
  •   Thompson, Fletcher W. (external author)
  •   Beirne, Stephen T.
  •   Wallace, Gordon G.
  •   Paull, Brett (external author)

Publication Date


  • 2016

Citation


  • Gupta, V., Talebi, M., Deverell, J., Sandron, S., Nesterenko, P. N., Heery, B., Thompson, F., Beirne, S., Wallace, G. G. & Paull, B. (2016). 3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography. Analytica Chimica Acta, 910 84-94.

Scopus Eid


  • 2-s2.0-84955579929

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/2040

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 84

End Page


  • 94

Volume


  • 910

Place Of Publication


  • Netherlands