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Exploring the properties of genetically engineered silk-elastin-like protein films

Journal Article


Abstract


  • Free standing films of a genetically engineered silk-elastin-like protein (SELP) were prepared using water and formic acid as solvents. Exposure to methanol-saturated air promoted the formation of aggregated β-strands rendering aqueous insolubility and improved the mechanical properties leading to a 10-fold increase in strain-to-failure. The films were optically clear with resistivity values similar to natural rubber and thermally stable up to 180 °C. Addition of glycerol showed to enhance the flexibility of SELP/glycerol films by interacting with SELP molecules through hydrogen bonding, interpenetrating between the polymer chains and granting more conformational freedom. This detailed characterization provides cues for future and unique applications using SELP based biopolymers.

UOW Authors


  •   Machado, Raul (external author)
  •   Da Costa, André (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Pereira, Ana (external author)
  •   Collins, Tony (external author)
  •   Rodríguez-Cabello, José C. (external author)
  •   Lanceros-Méndez, Senentxu (external author)
  •   Casal, Margarida (external author)

Publication Date


  • 2015

Citation


  • Machado, R., Da Costa, A., Sencadas, V., Pereira, A. Margarida., Collins, T., Rodríguez-Cabello, J. Carlos., Lanceros-Méndez, S. & Casal, M. (2015). Exploring the properties of genetically engineered silk-elastin-like protein films. Macromolecular Bioscience, 15 (12), 1698-1709.

Scopus Eid


  • 2-s2.0-84954468665

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/5130

Number Of Pages


  • 11

Start Page


  • 1698

End Page


  • 1709

Volume


  • 15

Issue


  • 12

Abstract


  • Free standing films of a genetically engineered silk-elastin-like protein (SELP) were prepared using water and formic acid as solvents. Exposure to methanol-saturated air promoted the formation of aggregated β-strands rendering aqueous insolubility and improved the mechanical properties leading to a 10-fold increase in strain-to-failure. The films were optically clear with resistivity values similar to natural rubber and thermally stable up to 180 °C. Addition of glycerol showed to enhance the flexibility of SELP/glycerol films by interacting with SELP molecules through hydrogen bonding, interpenetrating between the polymer chains and granting more conformational freedom. This detailed characterization provides cues for future and unique applications using SELP based biopolymers.

UOW Authors


  •   Machado, Raul (external author)
  •   Da Costa, André (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Pereira, Ana (external author)
  •   Collins, Tony (external author)
  •   Rodríguez-Cabello, José C. (external author)
  •   Lanceros-Méndez, Senentxu (external author)
  •   Casal, Margarida (external author)

Publication Date


  • 2015

Citation


  • Machado, R., Da Costa, A., Sencadas, V., Pereira, A. Margarida., Collins, T., Rodríguez-Cabello, J. Carlos., Lanceros-Méndez, S. & Casal, M. (2015). Exploring the properties of genetically engineered silk-elastin-like protein films. Macromolecular Bioscience, 15 (12), 1698-1709.

Scopus Eid


  • 2-s2.0-84954468665

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/5130

Number Of Pages


  • 11

Start Page


  • 1698

End Page


  • 1709

Volume


  • 15

Issue


  • 12