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Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation

Journal Article


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Abstract


  • The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.

UOW Authors


  •   Kozlowski, Rachel (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2015

Citation


  • Kozlowski, R. L., Mitchell, T. W. & Blanksby, S. J. (2015). Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation. European Journal of Mass Spectrometry, 21 (3), 191-200.

Scopus Eid


  • 2-s2.0-84952719834

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1630&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/608

Number Of Pages


  • 9

Start Page


  • 191

End Page


  • 200

Volume


  • 21

Issue


  • 3

Abstract


  • The differentiation of closely related lipid isomers is increasingly important to our evolving understanding of lipid biochemistry but it is equally challenging to contemporary chromatographic and mass spectral analyses. Recently, we described a novel ion-activation approach based on combining collision- with ozone-induced dissociation (CID/OzID) for the identification of the relative acyl chain substitution positions in glycerophospholipids. Here we demonstrate, for the first time, that CID/OzID can be effectively combined with reversed-phase chromatography to enable the separation and unambiguous identification of regioisomeric pairs of phosphatidylcholines that differ only in the arrangement of acyl chains on the glycerol backbone.

UOW Authors


  •   Kozlowski, Rachel (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2015

Citation


  • Kozlowski, R. L., Mitchell, T. W. & Blanksby, S. J. (2015). Separation and identification of phosphatidylcholine regioisomers by combining liquid chromatography with a fusion of collision-and ozone-induced dissociation. European Journal of Mass Spectrometry, 21 (3), 191-200.

Scopus Eid


  • 2-s2.0-84952719834

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1630&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/608

Number Of Pages


  • 9

Start Page


  • 191

End Page


  • 200

Volume


  • 21

Issue


  • 3