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Intersubject and interday variability in human tear and meibum lipidomes: a pilot study

Journal Article


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Abstract


  • Purpose

    Our aim was to quantitate day-to-day changes in the tear and meibum lipid profile of individual subjects in a pilot study of healthy humans.

    Methods

    Matched tear and meibum samples were obtained from four subjects on three consecutive days. Quantitative lipid profiles of human basal tears and meibum were compared using multivariate analysis by principal components.

    Results

    Substantial differences in the lipid profile between subjects were observed, while lipid profiles were steady across the three consecutive days of sampling. Multivariate principal component analysis demonstrated that lysophosphatidylcholine was the largest variant lipid class between subjects in tears, while wax esters comprised the most variation between subjects in meibum secretions.

    Conclusion

    Interday variability is shown to be much smaller than interpatient variability, suggesting that tears and meibum subjects both have unique profiles in humans.

UOW Authors


  •   Brown, Simon (external author)
  •   Kunnen, Carolina M. E. (external author)
  •   Papas, Eric B. (external author)
  •   Lazon de la Jara, Percy (external author)
  •   Willcox, Mark D. P. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2016

Citation


  • Brown, S. H. J., Kunnen, C. M. E., Papas, E. B., Lazon de la Jara, P., Willcox, M. D. P., Blanksby, S. J. & Mitchell, T. W. (2016). Intersubject and interday variability in human tear and meibum lipidomes: a pilot study. The Ocular Surface, 14 (1), 43-48.

Scopus Eid


  • 2-s2.0-84951926245

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1601&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/579

Number Of Pages


  • 5

Start Page


  • 43

End Page


  • 48

Volume


  • 14

Issue


  • 1

Abstract


  • Purpose

    Our aim was to quantitate day-to-day changes in the tear and meibum lipid profile of individual subjects in a pilot study of healthy humans.

    Methods

    Matched tear and meibum samples were obtained from four subjects on three consecutive days. Quantitative lipid profiles of human basal tears and meibum were compared using multivariate analysis by principal components.

    Results

    Substantial differences in the lipid profile between subjects were observed, while lipid profiles were steady across the three consecutive days of sampling. Multivariate principal component analysis demonstrated that lysophosphatidylcholine was the largest variant lipid class between subjects in tears, while wax esters comprised the most variation between subjects in meibum secretions.

    Conclusion

    Interday variability is shown to be much smaller than interpatient variability, suggesting that tears and meibum subjects both have unique profiles in humans.

UOW Authors


  •   Brown, Simon (external author)
  •   Kunnen, Carolina M. E. (external author)
  •   Papas, Eric B. (external author)
  •   Lazon de la Jara, Percy (external author)
  •   Willcox, Mark D. P. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2016

Citation


  • Brown, S. H. J., Kunnen, C. M. E., Papas, E. B., Lazon de la Jara, P., Willcox, M. D. P., Blanksby, S. J. & Mitchell, T. W. (2016). Intersubject and interday variability in human tear and meibum lipidomes: a pilot study. The Ocular Surface, 14 (1), 43-48.

Scopus Eid


  • 2-s2.0-84951926245

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1601&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/579

Number Of Pages


  • 5

Start Page


  • 43

End Page


  • 48

Volume


  • 14

Issue


  • 1