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Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Journal Article


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Abstract


  • This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored.

UOW Authors


  •   Goncalves, Sara (external author)
  •   Rodrigues, Ines P. (external author)
  •   Padrão, J (external author)
  •   Silva, J P. (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Lanceros-Méndez, Senentxu (external author)
  •   Girao, Henrique (external author)
  •   Gama, F M. (external author)
  •   Dourado, F (external author)
  •   Rodrigues, L R. (external author)

Publication Date


  • 2016

Citation


  • Goncalves, S., Rodrigues, I. Patricio., Padrão, J., Silva, J. Pedro., Sencadas, V., Lanceros-Méndez, S., Girao, H., Gama, F. M., Dourado, F. & Rodrigues, L. R. (2016). Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium. Colloids and Surfaces B: Biointerfaces, 139 1-9.

Scopus Eid


  • 2-s2.0-84949636357

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5874&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/4847

Number Of Pages


  • 8

Start Page


  • 1

End Page


  • 9

Volume


  • 139

Abstract


  • This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored.

UOW Authors


  •   Goncalves, Sara (external author)
  •   Rodrigues, Ines P. (external author)
  •   Padrão, J (external author)
  •   Silva, J P. (external author)
  •   Gomes da Silva Sencadas, Vitor
  •   Lanceros-Méndez, Senentxu (external author)
  •   Girao, Henrique (external author)
  •   Gama, F M. (external author)
  •   Dourado, F (external author)
  •   Rodrigues, L R. (external author)

Publication Date


  • 2016

Citation


  • Goncalves, S., Rodrigues, I. Patricio., Padrão, J., Silva, J. Pedro., Sencadas, V., Lanceros-Méndez, S., Girao, H., Gama, F. M., Dourado, F. & Rodrigues, L. R. (2016). Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium. Colloids and Surfaces B: Biointerfaces, 139 1-9.

Scopus Eid


  • 2-s2.0-84949636357

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5874&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/4847

Number Of Pages


  • 8

Start Page


  • 1

End Page


  • 9

Volume


  • 139