Skip to main content
placeholder image

Chlorogenic acid protects d-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice

Journal Article


Download full-text (Open Access)

Abstract


  • Context Oxidative stress and inflammation are implicated in the aging process and its related hepatic and renal function decline. Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in the human diet. Recently, CGA has shown in vivo and in vitro antioxidant properties.

    Objective The current study investigates the effects of protective effects of chlorogenic acid (CGA) on d-galactose-induced liver and kidney injury.

    Materials and methods Hepatic and renal injuries were induced in a mouse model by subcutaneously injection of d-galactose (d-gal; 100 mg/kg) once a day for 8 consecutive weeks and orally administered simultaneously with CGA included in the food (200 mg/kg of diet). The liver and renal functions were examined. Histological analyses of liver and kidney were done by haematoxylin and eosin staining. The oxidative stress markers and pro-inflammatory cytokines in the liver and the kidney were measured.

    Results CGA significantly reduced the serum aminotransferase, serum creatinine (SCr) and blood urea nitrogen (BUN) levels in d-gal mice (p <0.05). CGA also restored superoxide dismutase, catalase, and malondialdehyde levels and decreased glutathione content in the liver and kidney in d-gal mice (p <0.05). Improvements in liver and kidney were also noted in histopathological studies. CGA reduced tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) protein levels in the liver and kidney in d-gal mice (p <0.05).

    Discussion and conclusion These findings suggest that CGA attenuates d-gal-induced chronic liver and kidney injury and that this protection may be due to its antioxidative and anti-inflammatory activities.

Authors


  •   Feng, Yan (external author)
  •   Yu, Yinghua
  •   Wang, Shu-Ting (external author)
  •   Ren, Jing (external author)
  •   Camer, Danielle (external author)
  •   Hua, Yu-Zhou (external author)
  •   Zhang, Qian (external author)
  •   Huang, Jie (external author)
  •   Xue, Dan-Lu (external author)
  •   Zhang, Xiaofei (external author)
  •   Huang, Xu-Feng
  •   Liu, Yi (external author)

Publication Date


  • 2016

Citation


  • Feng, Y., Yu, Y., Wang, S., Ren, J., Camer, D., Hua, Y., Zhang, Q., Huang, J., Xue, D., Zhang, X., Huang, X. & Liu, Y. (2016). Chlorogenic acid protects d-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice. Pharmaceutical Biology, 54 (6), 1027-1034.

Scopus Eid


  • 2-s2.0-84958525998

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1913&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/889

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1027

End Page


  • 1034

Volume


  • 54

Issue


  • 6

Place Of Publication


  • United Kingdom

Abstract


  • Context Oxidative stress and inflammation are implicated in the aging process and its related hepatic and renal function decline. Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in the human diet. Recently, CGA has shown in vivo and in vitro antioxidant properties.

    Objective The current study investigates the effects of protective effects of chlorogenic acid (CGA) on d-galactose-induced liver and kidney injury.

    Materials and methods Hepatic and renal injuries were induced in a mouse model by subcutaneously injection of d-galactose (d-gal; 100 mg/kg) once a day for 8 consecutive weeks and orally administered simultaneously with CGA included in the food (200 mg/kg of diet). The liver and renal functions were examined. Histological analyses of liver and kidney were done by haematoxylin and eosin staining. The oxidative stress markers and pro-inflammatory cytokines in the liver and the kidney were measured.

    Results CGA significantly reduced the serum aminotransferase, serum creatinine (SCr) and blood urea nitrogen (BUN) levels in d-gal mice (p <0.05). CGA also restored superoxide dismutase, catalase, and malondialdehyde levels and decreased glutathione content in the liver and kidney in d-gal mice (p <0.05). Improvements in liver and kidney were also noted in histopathological studies. CGA reduced tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) protein levels in the liver and kidney in d-gal mice (p <0.05).

    Discussion and conclusion These findings suggest that CGA attenuates d-gal-induced chronic liver and kidney injury and that this protection may be due to its antioxidative and anti-inflammatory activities.

Authors


  •   Feng, Yan (external author)
  •   Yu, Yinghua
  •   Wang, Shu-Ting (external author)
  •   Ren, Jing (external author)
  •   Camer, Danielle (external author)
  •   Hua, Yu-Zhou (external author)
  •   Zhang, Qian (external author)
  •   Huang, Jie (external author)
  •   Xue, Dan-Lu (external author)
  •   Zhang, Xiaofei (external author)
  •   Huang, Xu-Feng
  •   Liu, Yi (external author)

Publication Date


  • 2016

Citation


  • Feng, Y., Yu, Y., Wang, S., Ren, J., Camer, D., Hua, Y., Zhang, Q., Huang, J., Xue, D., Zhang, X., Huang, X. & Liu, Y. (2016). Chlorogenic acid protects d-galactose-induced liver and kidney injury via antioxidation and anti-inflammation effects in mice. Pharmaceutical Biology, 54 (6), 1027-1034.

Scopus Eid


  • 2-s2.0-84958525998

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1913&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/889

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1027

End Page


  • 1034

Volume


  • 54

Issue


  • 6

Place Of Publication


  • United Kingdom