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Replisome speed determines the efficiency of the Tus-Ter replication termination barrier

Journal Article


Abstract


  • In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites1. Escherichia coli sets a ‘replication fork trap’ that allows the first arriving fork to enter but not to leave the terminus region2, 3, 4, 5. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction3, 4, 5, 6, 7. However, the efficiency of fork blockage by Tus–Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro8, 9. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus–Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

Publication Date


  • 2015

Published In


Citation


  • Elshenawy, M. M., Jergic, S., Xu, Z., Sobhy, M. A., Takahashi, M., Oakley, A. J., Dixon, N. E. & Hamdan, S. M. (2015). Replisome speed determines the efficiency of the Tus-Ter replication termination barrier. Nature, 525 (7569), 394-398.

Scopus Eid


  • 2-s2.0-84942047816

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/557

Number Of Pages


  • 4

Start Page


  • 394

End Page


  • 398

Volume


  • 525

Issue


  • 7569

Abstract


  • In all domains of life, DNA synthesis occurs bidirectionally from replication origins. Despite variable rates of replication fork progression, fork convergence often occurs at specific sites1. Escherichia coli sets a ‘replication fork trap’ that allows the first arriving fork to enter but not to leave the terminus region2, 3, 4, 5. The trap is set by oppositely oriented Tus-bound Ter sites that block forks on approach from only one direction3, 4, 5, 6, 7. However, the efficiency of fork blockage by Tus–Ter does not exceed 50% in vivo despite its apparent ability to almost permanently arrest replication forks in vitro8, 9. Here we use data from single-molecule DNA replication assays and structural studies to show that both polarity and fork-arrest efficiency are determined by a competition between rates of Tus displacement and rearrangement of Tus–Ter interactions that leads to blockage of slower moving replisomes by two distinct mechanisms. To our knowledge this is the first example where intrinsic differences in rates of individual replisomes have different biological outcomes.

Publication Date


  • 2015

Published In


Citation


  • Elshenawy, M. M., Jergic, S., Xu, Z., Sobhy, M. A., Takahashi, M., Oakley, A. J., Dixon, N. E. & Hamdan, S. M. (2015). Replisome speed determines the efficiency of the Tus-Ter replication termination barrier. Nature, 525 (7569), 394-398.

Scopus Eid


  • 2-s2.0-84942047816

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/557

Number Of Pages


  • 4

Start Page


  • 394

End Page


  • 398

Volume


  • 525

Issue


  • 7569