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Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes

Journal Article


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Abstract


  • The increase of antibiotic resistant microorganisms urged the development and synthesis of novel antimicrobial biomaterials to be employed in a broad range of applications, ranging from food packaging to medical devices. This work describes the production and characterization of a protein-based electrospun fibrous membranes bearing antimicrobial properties. Its composition is exclusively comprised of proteins, with fish gelatine as structural matrix and bovine lactoferrin (bLF) as the active antimicrobial agent. The bLF bactericidal effect was determined against clinical isolates of Escherichia coli and Staphylococcus aureus through microdilution assays. Two distinctive methods were used to incorporate bLF into the fish gelatine nanofibres: (i) as a filler in the electrospinning formulation with concentrations of 2, 5 and 10 (wt%), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and (ii) through adsorption in a solution with 40mgmL-1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively.

UOW Authors


  •   Padrão, J (external author)
  •   Machado, Raul (external author)
  •   Casal, Margarida (external author)
  •   Lanceros-Méndez, Senentxu (external author)
  •   Rodrigues, L R. (external author)
  •   Dourado, F (external author)
  •   Gomes da Silva Sencadas, Vitor

Publication Date


  • 2015

Citation


  • Padrão, J., Machado, R., Casal, M., Lanceros-Méndez, S., Rodrigues, L. R., Dourado, F. & Sencadas, V. (2015). Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules, 81 608-614.

Scopus Eid


  • 2-s2.0-84940879089

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5635&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/4614

Number Of Pages


  • 6

Start Page


  • 608

End Page


  • 614

Volume


  • 81

Abstract


  • The increase of antibiotic resistant microorganisms urged the development and synthesis of novel antimicrobial biomaterials to be employed in a broad range of applications, ranging from food packaging to medical devices. This work describes the production and characterization of a protein-based electrospun fibrous membranes bearing antimicrobial properties. Its composition is exclusively comprised of proteins, with fish gelatine as structural matrix and bovine lactoferrin (bLF) as the active antimicrobial agent. The bLF bactericidal effect was determined against clinical isolates of Escherichia coli and Staphylococcus aureus through microdilution assays. Two distinctive methods were used to incorporate bLF into the fish gelatine nanofibres: (i) as a filler in the electrospinning formulation with concentrations of 2, 5 and 10 (wt%), and cross-linked with glutaraldehyde vapour, in order to achieve stability in aqueous solution; and (ii) through adsorption in a solution with 40mgmL-1 bLF. Fourier transform infrared spectroscopy analysis showed that the structure of both proteins remained intact through the electrospinning blending and cross-linking procedure. Remarkable antibacterial properties were obtained with membranes containing 5% and 10% bLF with a bacterial reduction of approximately 90% and 100%, respectively.

UOW Authors


  •   Padrão, J (external author)
  •   Machado, Raul (external author)
  •   Casal, Margarida (external author)
  •   Lanceros-Méndez, Senentxu (external author)
  •   Rodrigues, L R. (external author)
  •   Dourado, F (external author)
  •   Gomes da Silva Sencadas, Vitor

Publication Date


  • 2015

Citation


  • Padrão, J., Machado, R., Casal, M., Lanceros-Méndez, S., Rodrigues, L. R., Dourado, F. & Sencadas, V. (2015). Antibacterial performance of bovine lactoferrin-fish gelatine electrospun membranes. International Journal of Biological Macromolecules, 81 608-614.

Scopus Eid


  • 2-s2.0-84940879089

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=5635&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/4614

Number Of Pages


  • 6

Start Page


  • 608

End Page


  • 614

Volume


  • 81