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Magnetic nanoparticles for “smart liposomes”

Journal Article


Abstract


  • Liposomal drug delivery systems (LDDSs) are promising tools used for the treatment of diseases where highly toxic pharmacological agents are administered. Currently, destabilising LDDSs by a specific stimulus at a target site remains a major challenge. The bacterial mechanosensitive channel of large conductance (MscL) presents an excellent candidate biomolecule that could be employed as a remotely controlled pore-forming nanovalve for triggered drug release from LDDSs. In this study, we developed superparamagnetic nanoparticles for activation of the MscL nanovalves by magnetic field. Synthesised CoFe2O4 nanoparticles with the radius less than 10 nm were labelled by SH groups for attachment to MscL. Activation of MscL by magnetic field with the nanoparticles attached was examined by the patch clamp technique showing that the number of activated channels under ramp pressure increased upon application of the magnetic field. In addition, we have not observed any cytotoxicity of the nanoparticles in human cultured cells. Our study suggests the possibility of using magnetic nanoparticles as a specific trigger for activation of MscL nanovalves for drug release in LDDSs.

Publication Date


  • 2015

Citation


  • Nakayama, Y., Mustapic, M., Ebrahimian, H., Wagner, P., Kim, J., Hossain, M. Al., Horvat, J. & Martinac, B. (2015). Magnetic nanoparticles for “smart liposomes”. European Biophysics Journal, 44 (8), 647-654.

Scopus Eid


  • 2-s2.0-84946473283

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1536

Number Of Pages


  • 7

Start Page


  • 647

End Page


  • 654

Volume


  • 44

Issue


  • 8

Abstract


  • Liposomal drug delivery systems (LDDSs) are promising tools used for the treatment of diseases where highly toxic pharmacological agents are administered. Currently, destabilising LDDSs by a specific stimulus at a target site remains a major challenge. The bacterial mechanosensitive channel of large conductance (MscL) presents an excellent candidate biomolecule that could be employed as a remotely controlled pore-forming nanovalve for triggered drug release from LDDSs. In this study, we developed superparamagnetic nanoparticles for activation of the MscL nanovalves by magnetic field. Synthesised CoFe2O4 nanoparticles with the radius less than 10 nm were labelled by SH groups for attachment to MscL. Activation of MscL by magnetic field with the nanoparticles attached was examined by the patch clamp technique showing that the number of activated channels under ramp pressure increased upon application of the magnetic field. In addition, we have not observed any cytotoxicity of the nanoparticles in human cultured cells. Our study suggests the possibility of using magnetic nanoparticles as a specific trigger for activation of MscL nanovalves for drug release in LDDSs.

Publication Date


  • 2015

Citation


  • Nakayama, Y., Mustapic, M., Ebrahimian, H., Wagner, P., Kim, J., Hossain, M. Al., Horvat, J. & Martinac, B. (2015). Magnetic nanoparticles for “smart liposomes”. European Biophysics Journal, 44 (8), 647-654.

Scopus Eid


  • 2-s2.0-84946473283

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1536

Number Of Pages


  • 7

Start Page


  • 647

End Page


  • 654

Volume


  • 44

Issue


  • 8