Dixon, Nicholas E.

Overview

Professor Nick Dixon holds a PhD in Biochemistry from the University of Queensland (1978). He was a Research Fellow with Prof Alan Sargeson (Research School of Chemistry, ANU) before being awarded C.J. Martin and Fulbright Fellowships to study with Nobel laureate Prof Arthur Kornberg at Stanford University, U.S.A. He returned to the John Curtin School of Medical Research at ANU as a Queen Elizabeth II Fellow in 1983, and was appointed as Fellow in Biological Chemistry at the Research School of Chemistry, ANU in 1986. He was subsequently promoted to Senior Fellow and Professor, before leaving ANU in 2006 to take up his present position as Professor of Biological Chemistry at the University of Wollongong. He was awarded an Australian Research Council Australian Professorial Fellowship in 2008, and established UOW’s Centre for Medical and Molecular Bioscience in 2010. He is currently an academic leader of Molecular Horizons, a new facility for molecular visualization to be opened in 2019.

Top Publications

Year	Title
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published in eLife
2016	The E. coli DNA Replication Fork
2016	Weak and Transient Protein Interactions Determined by Solid-State NMR Published in Angewandte Chemie International Edition
2015	Probing molecular choreography through single-molecule biochemistry Published in Nature Structural and Molecular Biology
2015	Bacterial sliding clamp inhibitors that mimic the sequential binding mechanism of endogenous linear motifs Published in Journal of Medicinal Chemistry
2015	Replisome speed determines the efficiency of the Tus-Ter replication termination barrier Published in Nature
2014	Loading dynamics of a sliding DNA clamp Published in Angewandte Chemie International Edition
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published in The EMBO Journal
2012	Backbone assignment of fully protonated solid proteins by 1H detection and ultrafast magic-angle-spinning NMR spectroscopy Published in Angewandte Chemie International Edition
2011	E. coli DNA replication in the absence of free β clamps Published in The EMBO Journal
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published in Microbiology and Molecular Biology Reviews
2010	Nanometer-scale distance measurements in proteins using Gd3+ spin labeling Published in Journal of the American Chemical Society
2008	Single-molecule studies of fork dynamics in Escherichia coli DNA replication Published in Nature Structural and Molecular Biology

Research Areas

Research Overview

Research Interests
- Biological chemistry
- Protein structure and function
- Protein-protein interactions in / design principles for multiprotein dynamic molecular machines
- The bacterial DNA replication machinery as a target for antibacterial drugs
- New technologies for protein chemistry

Available for Collaborative Projects

Head Of

School of Chemistry and Molecular Bioscience Transitional Head 2018 -
Centre for Medical and Molecular Bioscience Director 2010 - 2018

Selected Publications

Journal Article

Year	Title
2020	A Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome Published In Molecular Cell
2020	Development of a single-stranded DNA-binding protein fluorescent fusion toolbox Published In Nucleic Acids Research
2020	Genetic Encoding of para-Pentafluorosulfanyl Phenylalanine: A Highly Hydrophobic and Strongly Electronegative Group for Stable Protein Interactions Published In Journal of the American Chemical Society
2019	A gatekeeping function of the replicative polymerase controls pathway choice in the resolution of lesion-stalled replisomes Published In Proceedings of the National Academy of Sciences of the United States of America
2019	Nuclease dead Cas9 is a programmable roadblock for DNA replication Published In Scientific Reports
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2018	Bacterial replisomes Published In Current Opinion in Structural Biology
2018	Crystal structures and biochemical characterization of DNA sliding clamps from three Gram-negative bacterial pathogens. Published In Journal of Structural Biology
2018	Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication Published In Analytical Biochemistry
2018	Dynamics of Proofreading by the E. coli Pol III Replicase Published In Cell Chemical Biology
2018	Fragment-based discovery of inhibitors of the bacterial DnaG-SSB interaction Published In Antibiotics
2018	Rational Design of a 310-Helical PIP-Box Mimetic Targeting PCNA, the Human Sliding Clamp Published In Chemistry: A European Journal
2018	Structure-activity relationships of pyrazole-4-carbodithioates as antibacterials against methicillin–resistant Staphylococcus aureus Published In Bioorganic and Medicinal Chemistry Letters
2018	What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments Published In Critical Reviews in Biochemistry and Molecular Biology
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2016	Exchange between Escherichia coli polymerases II and III on a processivity clamp Published In Nucleic Acids Research
2016	NMR spectroscopic assignment of backbone and side-chain protons in fully protonated proteins: microcrystals, sedimented assemblies, and amyloid fibrils Published In Angewandte Chemie International Edition
2016	Weak and Transient Protein Interactions Determined by Solid-State NMR Published In Angewandte Chemie International Edition
2015	A single-molecule reconstitution of translesion synthesis and competition between DNA polymerases Published In The FASEB Journal
2015	An investigation into the interactions of gold nanoparticles and anti-arthritic drugs with macrophages, and their reactivity towards thioredoxin reductase Published In Journal of Inorganic Biochemistry
2015	Bacterial sliding clamp inhibitors that mimic the sequential binding mechanism of endogenous linear motifs Published In Journal of Medicinal Chemistry
2015	Probing molecular choreography through single-molecule biochemistry Published In Nature Structural and Molecular Biology
2015	Protein residue linking in a single spectrum for magic-angle spinning NMR assignment Published In Journal of Biomolecular NMR
2015	Replisome speed determines the efficiency of the Tus-Ter replication termination barrier Published In Nature
2015	Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis Published In Nature Communications
2015	Strand separation establishes a sustained lock at the Tus-Ter replication fork barrier Published In Nature Chemical Biology
2015	Two mechanisms coordinate replication termination by the Escherichia coli Tus-Ter complex Published In Nucleic Acids Research
2014	AtfA, a new factor in global regulation of transcription in Acinetobacter spp Published In Molecular Microbiology
2014	Bound or free: interaction of the C-terminal domain of Escherichia coli single-stranded DNA-binding protein (SSB) with the tetrameric core of SSB Published In Biochemistry
2014	DNA replication is the target for the antibacterial effects of nonsteroidal anti-inflammatory drugs Published In Chemistry and Biology
2014	Discovery of lead compounds targeting the bacterial sliding clamp using a fragment-based approach Published In Journal of Medicinal Chemistry
2014	Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein determined by nuclear magnetic resonance spectroscopy Published In Nucleic Acids Research
2014	Loading dynamics of a sliding DNA clamp Published In Angewandte Chemie International Edition
2014	Polymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis Published In Proceedings of the National Academy of Sciences of USA
2014	Roadblocks on the E.Coli genome: the Workings of a molecular mouse trap at the single-molecule level Published In Biophysical Journal
2014	Structure and function of a spectrin-like regulator of bacterial cytokinesis Published In Nature Communications
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published In The EMBO Journal
2013	Escherichia coli single-stranded DNA-binding protein: nanoESI-MS studies of salt-modulated subunit exchange and DNA binding transactions Published In Journal of the American Society for Mass Spectrometry
2013	Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain Published In Nucleic Acids Research
2013	Replicative DNA polymerases Published In Cold Spring Harbor Perspectives in Biology
2012	A new role for the proofreader in bacterial DNA replication Published In The FASEB Journal
2012	Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target Published In Current Drug Targets
2012	Backbone assignment of fully protonated solid proteins by 1H detection and ultrafast magic-angle-spinning NMR spectroscopy Published In Angewandte Chemie International Edition
2012	Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which Mycoplasma hyopneumoniae regulates surface topography Published In mBio
2012	High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites Published In Biochemical and Biophysical Research Communications
2012	Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface Published In Cellular Microbiology
2012	Priming the engine of DNA synthesis Published In Structure
2012	Ultra fast MAS solid-state NMR studies of protein-protein interactions in the bacterial replisome Published In The Federation of European Biochemical Societies (FEBS) Journal
2011	Binding inhibitors of the bacterial sliding clamp by design Published In Journal of Medicinal Chemistry
2011	E. coli DNA replication in the absence of free β clamps Published In The EMBO Journal
2011	EX1 hydrogen-deuterium exchange in an all-helical protein and its cyclized derivative at neutral pH Published In International Journal of Mass Spectrometry
2011	Improving a natural enzyme activity through incorporation of unnatural amino acids Published In Journal of the American Chemical Society
2011	Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis Published In Chemical Communications
2011	Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae Published In Journal of Biological Chemistry
2010	A Single subunit directs the assembly of the escherichia coli DNA sliding clamp loader Published In Structure
2010	A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia Published In Journal of Biological Chemistry
2010	Chaperonin-encapsulation of proteins for NMR Published In BBA: Proteins and Proteomics
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published In Microbiology and Molecular Biology Reviews
2010	Nanometer-scale distance measurements in proteins using Gd3+ spin labeling Published In Journal of the American Chemical Society
2010	Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia Published In Molecular Microbiology
2010	The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs Published In The Federation of European Biochemical Societies (FEBS) Journal
2010	Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system Published In Molecular Biosystems
2009	A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis Dnal helicase loader Published In Nucleic Acids Research
2009	Cell-free synthesis and combinatorial selective 15 N-labeling of the cytotoxic protein amoebapore A from Entamoeba histolytica Published In Protein Expression and Purification
2009	DNA Replication Prime-time looping Published In Nature
2009	Defining the structural basis of human plasminogen binding by streptococcal surface enolase Published In Journal of Biological Chemistry
2009	Real-time single-molecule observation of rolling-circle DNA replication Published In Nucleic Acids Research
2009	Site-specific covalent attachment of DNA to proteins using a photoactivatable Tus-Ter complex Published In Chemical Communications
2009	Synthesis and applications of covalent protein-DNA conjugates Published In Australian Journal of Chemistry: an international journal for chemical science
2008	Characterization of gibberellin receptor mutants of barley (Hordeum vulgare L.) Published In Molecular Plant
2008	Hydrolysis of the 5'-p-nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli, Mycobacterium smegmatis, and human Published In Protein Expression and Purification
2008	Single-molecule studies of fork dynamics in Escherichia coli DNA replication Published In Nature Structural and Molecular Biology
2008	The proofreading exonuclease subunit E of Escherichia coli DNA polymerase III is tethered to the polymerase subunit a via a flexible linker Published In Nucleic Acids Research
2007	Assignment of paramagnetic 15N-HSQC spectra by heteronuclear exchange spectroscopy Published In Journal of Biomolecular NMR
2007	Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies Published In Angewandte Chemie International Edition
2007	Effect of protein stabilization on charge state distribution in positive- and negative ion electrospray ionization mass spectra Published In Journal of the American Society for Mass Spectrometry
2007	Measurement of dissociation constants of high-molecular weight protein-protein complexes by transferred 15N-relaxation Published In Journal of Biomolecular NMR
2007	NMR detection of protein 15N-spins near paramagnetic lanthanide ions Published In Journal of the American Chemical Society
2007	Sequence-specific and stereospecific assignment of methyl groups using paramagnetic lanthanides Published In Journal of the American Chemical Society
2007	Solution structure of Domains IVa and V of the tau subunit of Escherichia coli DNA polymerase III and interaction with the alpha subunit Published In Nucleic Acids Research
2007	The unstructured C-terminus of the tau subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the alpha subunit Published In Nucleic Acids Research

Chapter

Year	Title
2016	The E. coli DNA Replication Fork Published In DNA Replication Across Taxa Publisher Cambridge, United States
2010	Hybrid methods for protein structure prediction Published In Introduction to Protein Structure Prediction: Methods and Algorithms Publisher 111 River Street, Hoboken, NJ 07030
2008	Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy Published In Structural proteomics : high-throughput methods Publisher Totowa, NJ, USA

Conference Paper

Year	Title
2010	Structure, dynamics and function of replisomal Protein complex Published In 9th AINSE / ANBUG Neutron Scattering Symposium: AANSS 2010

Investigator On

Year	Title
2021	Roadblocks in DNA replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2021	Structures to Solve Conflicts of DNA Replication and RNA Transcription Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2019 - 2021	Creation and Repair of Postreplicative DNA Gaps Awarded by: National Institute of Health (NIH) Funding Scheme: Grant
2019	Antimicrobial Research Taskforce Awarded by: NUW Alliance Funding Scheme: Grant
2018	Ultrafast magic angle spinning solid-state NMR capability Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2017	A High Field Pulse EPR Facility - Enhanced Sensitivity and Resolution Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2016	Distributed Facility for Fragment Based Drug Discovery Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2016	SEC-SAXS for protein solution conformations in (1) the replisome and (2) Awarded by: Australian Synchrotron Company P/L Funding Scheme: Travel Grant
2016	Superresolution fluorescence imaging in microbiology Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2014	Regional flow cytometry facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2014	Size-Exchusion Chromatography Coupled SAXS of Macromolecular Complexes: the Replisome and the Divisome Awarded by: Australian Synchrotron Research Program Funding Scheme: Grant
2013	Facilities for protein purification and characterisation Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2010 - 2013	Universal Ultrasensitive Diagnostics Platform Alliance Awarded by: Queensland Government Funding Scheme: National & International Research Alliances Program (NIRAP)
2012	Integrated facility for confocal imaging and single molecule flourescence analysis Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2012	Scanspeed 1248R benchtop refrigerated centrifuge etc. Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2012	Targeting Nucleic Acid Synthesis and Cell Division in Gram-Negative Bacterial Pathogens Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2011	Establishment of a comprehensive regional biophysical analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2011	Regional facility for macomolecular X-ray crystallography Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2011	Targeting lagging strand DNA replication in model and pathogenic bacteria Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2010	Facility for real-time study of molecular interactions Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2010	Federated single crystal x-ray structural analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2010	Regional facility for molecular characterisation and mapping Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2010	Single molecule view of DNA replication Awarded by: KAUST - King Abdullah University of Science and Technology Funding Scheme: Global Collaborative Research
2009	Facilities for protein expression and purification Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2009	Mapping protein contacts and conformational changes in macromolecular assemblies Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2009	Microbial and cellular imaging and analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2009	Regional facility for real time analysis of molecular interactions Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2008	A stable protein: DNA complex for development of ultrasensitive diagnostics in multiplex format Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2008	Facility for protein structure determination by X-ray crystallography Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2008	Functional Dissection of the Bacterial Replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2008	Mass Spectrometric Investigations of Conformation and Dynamics of Biological Complexes Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage International
2008	New techniques for structural biology and directed molecular evolution Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Projects
2008	X-ray crystallography resource for membrane proteins and large macromolecular complexes. Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2007	Avestin EmulsiFlex TM-C5 Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2007	Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2007	Study of relationships between function, structure and dynamics of biological molecules by neutron scattering Awarded by: Australian Institute of Nuclear Science and Engineering (AINSE) Funding Scheme: Research Fellowships
	A functional dissection of the bacterial replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Functional dissection of the bacterial replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Molecular Interactions with an Antibiotic Target in DNA Replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Rescuing Stalled Replisomes by Accessory Helicases at the Single-Molecule Level Awarded by: KAUST - King Abdullah University of Science and Technology Funding Scheme: Competitive Research Grant Scheme (CRG)
	X-ray facility for protein crystallography Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	Single-molecule studies of Saccharomyces cerevisiae DNA replication	Natarajan, Varsha
Doctor of Philosophy	Plant gene therapy - Development of a bacterial delivery system for RNAi in plants	Goodfellow, Simon
Doctor of Philosophy	Single-molecule visualisation of engine choreography during Escherichia Coli DNA Replication	Spinks, Richard
Doctor of Philosophy	Single-molecule studies of the bacterial replisome	Mueller, Stefan
Master of Research -SMAH	Cryo-EM Structures of E. coli Clamp Loader Complexes	Pradhan, Alok
Doctor of Philosophy	Characterisation of novel arsenic resistance genes	Scifleet, James

Full Name

Prof. Nicholas Edward Dixon

Mailing Address

School of Chemistry, Bldg 18.

Northfields Avenue

University of Wollongong

NSW

2522

Australia

Geographic Location

University of Wollongong

Orcid Id

http://orcid.org/0000-0002-5958-6945 (confirmed)

Web Of Science Researcher Id

Recearcher ID: A-1226-2008

Scopus Id

Scopus ID: 35510468100

Top Publications

Year	Title
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published in eLife
2016	The E. coli DNA Replication Fork
2016	Weak and Transient Protein Interactions Determined by Solid-State NMR Published in Angewandte Chemie International Edition
2015	Probing molecular choreography through single-molecule biochemistry Published in Nature Structural and Molecular Biology
2015	Bacterial sliding clamp inhibitors that mimic the sequential binding mechanism of endogenous linear motifs Published in Journal of Medicinal Chemistry
2015	Replisome speed determines the efficiency of the Tus-Ter replication termination barrier Published in Nature
2014	Loading dynamics of a sliding DNA clamp Published in Angewandte Chemie International Edition
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published in The EMBO Journal
2012	Backbone assignment of fully protonated solid proteins by 1H detection and ultrafast magic-angle-spinning NMR spectroscopy Published in Angewandte Chemie International Edition
2011	E. coli DNA replication in the absence of free β clamps Published in The EMBO Journal
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published in Microbiology and Molecular Biology Reviews
2010	Nanometer-scale distance measurements in proteins using Gd3+ spin labeling Published in Journal of the American Chemical Society
2008	Single-molecule studies of fork dynamics in Escherichia coli DNA replication Published in Nature Structural and Molecular Biology

Research Areas

Research Overview

Research Interests
- Biological chemistry
- Protein structure and function
- Protein-protein interactions in / design principles for multiprotein dynamic molecular machines
- The bacterial DNA replication machinery as a target for antibacterial drugs
- New technologies for protein chemistry

Head Of

School of Chemistry and Molecular Bioscience Transitional Head 2018 -
Centre for Medical and Molecular Bioscience Director 2010 - 2018

Selected Publications

Journal Article

Year	Title
2020	A Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome Published In Molecular Cell
2020	Development of a single-stranded DNA-binding protein fluorescent fusion toolbox Published In Nucleic Acids Research
2020	Genetic Encoding of para-Pentafluorosulfanyl Phenylalanine: A Highly Hydrophobic and Strongly Electronegative Group for Stable Protein Interactions Published In Journal of the American Chemical Society
2019	A gatekeeping function of the replicative polymerase controls pathway choice in the resolution of lesion-stalled replisomes Published In Proceedings of the National Academy of Sciences of the United States of America
2019	Nuclease dead Cas9 is a programmable roadblock for DNA replication Published In Scientific Reports
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2018	Bacterial replisomes Published In Current Opinion in Structural Biology
2018	Crystal structures and biochemical characterization of DNA sliding clamps from three Gram-negative bacterial pathogens. Published In Journal of Structural Biology
2018	Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication Published In Analytical Biochemistry
2018	Dynamics of Proofreading by the E. coli Pol III Replicase Published In Cell Chemical Biology
2018	Fragment-based discovery of inhibitors of the bacterial DnaG-SSB interaction Published In Antibiotics
2018	Rational Design of a 310-Helical PIP-Box Mimetic Targeting PCNA, the Human Sliding Clamp Published In Chemistry: A European Journal
2018	Structure-activity relationships of pyrazole-4-carbodithioates as antibacterials against methicillin–resistant Staphylococcus aureus Published In Bioorganic and Medicinal Chemistry Letters
2018	What is all this fuss about Tus? Comparison of recent findings from biophysical and biochemical experiments Published In Critical Reviews in Biochemistry and Molecular Biology
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2016	Exchange between Escherichia coli polymerases II and III on a processivity clamp Published In Nucleic Acids Research
2016	NMR spectroscopic assignment of backbone and side-chain protons in fully protonated proteins: microcrystals, sedimented assemblies, and amyloid fibrils Published In Angewandte Chemie International Edition
2016	Weak and Transient Protein Interactions Determined by Solid-State NMR Published In Angewandte Chemie International Edition
2015	A single-molecule reconstitution of translesion synthesis and competition between DNA polymerases Published In The FASEB Journal
2015	An investigation into the interactions of gold nanoparticles and anti-arthritic drugs with macrophages, and their reactivity towards thioredoxin reductase Published In Journal of Inorganic Biochemistry
2015	Bacterial sliding clamp inhibitors that mimic the sequential binding mechanism of endogenous linear motifs Published In Journal of Medicinal Chemistry
2015	Probing molecular choreography through single-molecule biochemistry Published In Nature Structural and Molecular Biology
2015	Protein residue linking in a single spectrum for magic-angle spinning NMR assignment Published In Journal of Biomolecular NMR
2015	Replisome speed determines the efficiency of the Tus-Ter replication termination barrier Published In Nature
2015	Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis Published In Nature Communications
2015	Strand separation establishes a sustained lock at the Tus-Ter replication fork barrier Published In Nature Chemical Biology
2015	Two mechanisms coordinate replication termination by the Escherichia coli Tus-Ter complex Published In Nucleic Acids Research
2014	AtfA, a new factor in global regulation of transcription in Acinetobacter spp Published In Molecular Microbiology
2014	Bound or free: interaction of the C-terminal domain of Escherichia coli single-stranded DNA-binding protein (SSB) with the tetrameric core of SSB Published In Biochemistry
2014	DNA replication is the target for the antibacterial effects of nonsteroidal anti-inflammatory drugs Published In Chemistry and Biology
2014	Discovery of lead compounds targeting the bacterial sliding clamp using a fragment-based approach Published In Journal of Medicinal Chemistry
2014	Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein determined by nuclear magnetic resonance spectroscopy Published In Nucleic Acids Research
2014	Loading dynamics of a sliding DNA clamp Published In Angewandte Chemie International Edition
2014	Polymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis Published In Proceedings of the National Academy of Sciences of USA
2014	Roadblocks on the E.Coli genome: the Workings of a molecular mouse trap at the single-molecule level Published In Biophysical Journal
2014	Structure and function of a spectrin-like regulator of bacterial cytokinesis Published In Nature Communications
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published In The EMBO Journal
2013	Escherichia coli single-stranded DNA-binding protein: nanoESI-MS studies of salt-modulated subunit exchange and DNA binding transactions Published In Journal of the American Society for Mass Spectrometry
2013	Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain Published In Nucleic Acids Research
2013	Replicative DNA polymerases Published In Cold Spring Harbor Perspectives in Biology
2012	A new role for the proofreader in bacterial DNA replication Published In The FASEB Journal
2012	Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target Published In Current Drug Targets
2012	Backbone assignment of fully protonated solid proteins by 1H detection and ultrafast magic-angle-spinning NMR spectroscopy Published In Angewandte Chemie International Edition
2012	Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a mechanism by which Mycoplasma hyopneumoniae regulates surface topography Published In mBio
2012	High-yield cell-free protein synthesis for site-specific incorporation of unnatural amino acids at two sites Published In Biochemical and Biophysical Research Communications
2012	Mhp182 (P102) binds fibronectin and contributes to the recruitment of plasmin(ogen) to the Mycoplasma hyopneumoniae cell surface Published In Cellular Microbiology
2012	Priming the engine of DNA synthesis Published In Structure
2012	Ultra fast MAS solid-state NMR studies of protein-protein interactions in the bacterial replisome Published In The Federation of European Biochemical Societies (FEBS) Journal
2011	Binding inhibitors of the bacterial sliding clamp by design Published In Journal of Medicinal Chemistry
2011	E. coli DNA replication in the absence of free β clamps Published In The EMBO Journal
2011	EX1 hydrogen-deuterium exchange in an all-helical protein and its cyclized derivative at neutral pH Published In International Journal of Mass Spectrometry
2011	Improving a natural enzyme activity through incorporation of unnatural amino acids Published In Journal of the American Chemical Society
2011	Incorporation of chlorinated analogues of aliphatic amino acids during cell-free protein synthesis Published In Chemical Communications
2011	Mhp107 is a member of the multifunctional adhesin family of Mycoplasma hyopneumoniae Published In Journal of Biological Chemistry
2010	A Single subunit directs the assembly of the escherichia coli DNA sliding clamp loader Published In Structure
2010	A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen, and swine respiratory cilia Published In Journal of Biological Chemistry
2010	Chaperonin-encapsulation of proteins for NMR Published In BBA: Proteins and Proteomics
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published In Microbiology and Molecular Biology Reviews
2010	Nanometer-scale distance measurements in proteins using Gd3+ spin labeling Published In Journal of the American Chemical Society
2010	Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia Published In Molecular Microbiology
2010	The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs Published In The Federation of European Biochemical Societies (FEBS) Journal
2010	Ultrasensitive detection of antibodies using a new Tus-Ter-lock immunoPCR system Published In Molecular Biosystems
2009	A novel zinc-binding fold in the helicase interaction domain of the Bacillus subtilis Dnal helicase loader Published In Nucleic Acids Research
2009	Cell-free synthesis and combinatorial selective 15 N-labeling of the cytotoxic protein amoebapore A from Entamoeba histolytica Published In Protein Expression and Purification
2009	DNA Replication Prime-time looping Published In Nature
2009	Defining the structural basis of human plasminogen binding by streptococcal surface enolase Published In Journal of Biological Chemistry
2009	Real-time single-molecule observation of rolling-circle DNA replication Published In Nucleic Acids Research
2009	Site-specific covalent attachment of DNA to proteins using a photoactivatable Tus-Ter complex Published In Chemical Communications
2009	Synthesis and applications of covalent protein-DNA conjugates Published In Australian Journal of Chemistry: an international journal for chemical science
2008	Characterization of gibberellin receptor mutants of barley (Hordeum vulgare L.) Published In Molecular Plant
2008	Hydrolysis of the 5'-p-nitrophenyl ester of TMP by oligoribonucleases (ORN) from Escherichia coli, Mycobacterium smegmatis, and human Published In Protein Expression and Purification
2008	Single-molecule studies of fork dynamics in Escherichia coli DNA replication Published In Nature Structural and Molecular Biology
2008	The proofreading exonuclease subunit E of Escherichia coli DNA polymerase III is tethered to the polymerase subunit a via a flexible linker Published In Nucleic Acids Research
2007	Assignment of paramagnetic 15N-HSQC spectra by heteronuclear exchange spectroscopy Published In Journal of Biomolecular NMR
2007	Cell-free transcription/translation from PCR-amplified DNA for high-throughput NMR studies Published In Angewandte Chemie International Edition
2007	Effect of protein stabilization on charge state distribution in positive- and negative ion electrospray ionization mass spectra Published In Journal of the American Society for Mass Spectrometry
2007	Measurement of dissociation constants of high-molecular weight protein-protein complexes by transferred 15N-relaxation Published In Journal of Biomolecular NMR
2007	NMR detection of protein 15N-spins near paramagnetic lanthanide ions Published In Journal of the American Chemical Society
2007	Sequence-specific and stereospecific assignment of methyl groups using paramagnetic lanthanides Published In Journal of the American Chemical Society
2007	Solution structure of Domains IVa and V of the tau subunit of Escherichia coli DNA polymerase III and interaction with the alpha subunit Published In Nucleic Acids Research
2007	The unstructured C-terminus of the tau subunit of Escherichia coli DNA polymerase III holoenzyme is the site of interaction with the alpha subunit Published In Nucleic Acids Research

Chapter

Year	Title
2016	The E. coli DNA Replication Fork Published In DNA Replication Across Taxa Publisher Cambridge, United States
2010	Hybrid methods for protein structure prediction Published In Introduction to Protein Structure Prediction: Methods and Algorithms Publisher 111 River Street, Hoboken, NJ 07030
2008	Cell-Free Protein Synthesis for Analysis by NMR Spectroscopy Published In Structural proteomics : high-throughput methods Publisher Totowa, NJ, USA

Conference Paper

Year	Title
2010	Structure, dynamics and function of replisomal Protein complex Published In 9th AINSE / ANBUG Neutron Scattering Symposium: AANSS 2010

Investigator On

Year	Title
2021	Roadblocks in DNA replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2021	Structures to Solve Conflicts of DNA Replication and RNA Transcription Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2019 - 2021	Creation and Repair of Postreplicative DNA Gaps Awarded by: National Institute of Health (NIH) Funding Scheme: Grant
2019	Antimicrobial Research Taskforce Awarded by: NUW Alliance Funding Scheme: Grant
2018	Ultrafast magic angle spinning solid-state NMR capability Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2017	A High Field Pulse EPR Facility - Enhanced Sensitivity and Resolution Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2016	Distributed Facility for Fragment Based Drug Discovery Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2016	SEC-SAXS for protein solution conformations in (1) the replisome and (2) Awarded by: Australian Synchrotron Company P/L Funding Scheme: Travel Grant
2016	Superresolution fluorescence imaging in microbiology Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2014	Regional flow cytometry facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2014	Size-Exchusion Chromatography Coupled SAXS of Macromolecular Complexes: the Replisome and the Divisome Awarded by: Australian Synchrotron Research Program Funding Scheme: Grant
2013	Facilities for protein purification and characterisation Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2010 - 2013	Universal Ultrasensitive Diagnostics Platform Alliance Awarded by: Queensland Government Funding Scheme: National & International Research Alliances Program (NIRAP)
2012	Integrated facility for confocal imaging and single molecule flourescence analysis Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2012	Scanspeed 1248R benchtop refrigerated centrifuge etc. Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2012	Targeting Nucleic Acid Synthesis and Cell Division in Gram-Negative Bacterial Pathogens Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2011	Establishment of a comprehensive regional biophysical analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2011	Regional facility for macomolecular X-ray crystallography Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2011	Targeting lagging strand DNA replication in model and pathogenic bacteria Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2010	Facility for real-time study of molecular interactions Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2010	Federated single crystal x-ray structural analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2010	Regional facility for molecular characterisation and mapping Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2010	Single molecule view of DNA replication Awarded by: KAUST - King Abdullah University of Science and Technology Funding Scheme: Global Collaborative Research
2009	Facilities for protein expression and purification Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2009	Mapping protein contacts and conformational changes in macromolecular assemblies Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2009	Microbial and cellular imaging and analysis facility Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2009	Regional facility for real time analysis of molecular interactions Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2008	A stable protein: DNA complex for development of ultrasensitive diagnostics in multiplex format Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2008	Facility for protein structure determination by X-ray crystallography Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2008	Functional Dissection of the Bacterial Replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2008	Mass Spectrometric Investigations of Conformation and Dynamics of Biological Complexes Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage International
2008	New techniques for structural biology and directed molecular evolution Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Projects
2008	X-ray crystallography resource for membrane proteins and large macromolecular complexes. Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)
2007	Avestin EmulsiFlex TM-C5 Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2007	Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2007	Study of relationships between function, structure and dynamics of biological molecules by neutron scattering Awarded by: Australian Institute of Nuclear Science and Engineering (AINSE) Funding Scheme: Research Fellowships
	A functional dissection of the bacterial replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Functional dissection of the bacterial replisome Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Molecular Interactions with an Antibiotic Target in DNA Replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
	Rescuing Stalled Replisomes by Accessory Helicases at the Single-Molecule Level Awarded by: KAUST - King Abdullah University of Science and Technology Funding Scheme: Competitive Research Grant Scheme (CRG)
	X-ray facility for protein crystallography Awarded by: Australian Research Council (ARC) Funding Scheme: Linkage Infrastructure Equipment & Facilities (LIEF)

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	Single-molecule studies of Saccharomyces cerevisiae DNA replication	Natarajan, Varsha
Doctor of Philosophy	Plant gene therapy - Development of a bacterial delivery system for RNAi in plants	Goodfellow, Simon
Doctor of Philosophy	Single-molecule visualisation of engine choreography during Escherichia Coli DNA Replication	Spinks, Richard
Doctor of Philosophy	Single-molecule studies of the bacterial replisome	Mueller, Stefan
Master of Research -SMAH	Cryo-EM Structures of E. coli Clamp Loader Complexes	Pradhan, Alok
Doctor of Philosophy	Characterisation of novel arsenic resistance genes	Scifleet, James

Full Name

Prof. Nicholas Edward Dixon

Mailing Address

School of Chemistry, Bldg 18.

Northfields Avenue

University of Wollongong

NSW

2522

Australia

Geographic Location

University of Wollongong

Orcid Id

http://orcid.org/0000-0002-5958-6945 (confirmed)

Web Of Science Researcher Id

Recearcher ID: A-1226-2008

Scopus Id

Scopus ID: 35510468100

Overview

Overview

Top Publications

Research Areas

Research Overview

Affiliation

Available for Collaborative Projects

Head Of

Publications

Selected Publications

Journal Article

Chapter

Conference Paper

Funding

Investigator On

Supervision

Advisees

Graduate Advising Relationship

Contact

Full Name

Mailing Address

Geographic Location

Identity

Orcid Id

Web Of Science Researcher Id

Scopus Id

Overview

Top Publications

Research Areas

Research Overview

Affiliation

Head Of

Publications

Selected Publications

Journal Article

Chapter

Conference Paper

Funding

Investigator On

Supervision

Advisees

Graduate Advising Relationship

Contact

Full Name

Mailing Address

Geographic Location

Identity

Orcid Id

Web Of Science Researcher Id

Scopus Id

Visualisations

Research Areas