Spenkelink, Lisanne

Spenkelink, Lisanne Dr.

UOW PERL Fellow

lisanne@uow.edu.au

0242215748

Top Publications

Year	Title
2020	Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication Published in Molecular Cell
2018	Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging Published in Journal of Controlled Release
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published in eLife

Research Areas

0601 - BIOCHEMISTRY AND CELL BIOLOGY

Selected Publications

Journal Article

Year	Title
2021	Biology on track: Single-molecule visualisation of protein dynamics on linear DNA substrates Published In Essays in Biochemistry
2021	DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies Published In Nucleic Acids Research
2021	Single-Molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes
2021	Single-Molecule Insights Into the Dynamics of Replicative Helicases Published In Frontiers in Molecular Biosciences
2020	Design of customizable long linear DNA substrates with controlled end modifications for single-molecule studies Published In Analytical Biochemistry
2020	Development of a single-stranded DNA-binding protein fluorescent fusion toolbox Published In Nucleic Acids Research
2020	Replisome bypass of a protein-based R-loop block by Pif1 Published In Proceedings of the National Academy of Sciences of the United States of America
2020	Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication Published In Molecular Cell
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2019	Tunability of DNA polymerase stability during eukaryotic DNA replication
2019	When proteins play tag: the dynamic nature of the replisome Published In Biophysical Reviews
2018	Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging Published In Journal of Controlled Release
2017	Single-molecule visualization of Saccharomyces cerevisiae leading-strand synthesis reveals dynamic interaction between MTC and the replisome Published In Proceedings of the National Academy of Sciences of USA
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2017	Watching cellular machinery in action, one molecule at a time Published In The Journal of Cell Biology
2015	Single-molecule imaging at high fluorophore concentrations by local activation of dye Published In Biophysical Journal

Investigator On

Year	Title
2022 - 2026	Rapid evolution of a genome editing tool Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Investigator Grants

Other Research Activities

PERL Fellowship UOW PERL Fellow 2021 - 2022

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	Functional Implications of RarA at the Escherichia Coli Replication Fork
Doctor of Philosophy	Single-molecule studies of the bacterial replisome	Mueller, Stefan

Top Publications

Year	Title
2020	Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication Published in Molecular Cell
2018	Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging Published in Journal of Controlled Release
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published in eLife

Research Areas

0601 - BIOCHEMISTRY AND CELL BIOLOGY

Selected Publications

Journal Article

Year	Title
2021	Biology on track: Single-molecule visualisation of protein dynamics on linear DNA substrates Published In Essays in Biochemistry
2021	DnaB helicase dynamics in bacterial DNA replication resolved by single-molecule studies Published In Nucleic Acids Research
2021	Single-Molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular Complexes
2021	Single-Molecule Insights Into the Dynamics of Replicative Helicases Published In Frontiers in Molecular Biosciences
2020	Design of customizable long linear DNA substrates with controlled end modifications for single-molecule studies Published In Analytical Biochemistry
2020	Development of a single-stranded DNA-binding protein fluorescent fusion toolbox Published In Nucleic Acids Research
2020	Replisome bypass of a protein-based R-loop block by Pif1 Published In Proceedings of the National Academy of Sciences of the United States of America
2020	Tunability of DNA Polymerase Stability during Eukaryotic DNA Replication Published In Molecular Cell
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2019	Tunability of DNA polymerase stability during eukaryotic DNA replication
2019	When proteins play tag: the dynamic nature of the replisome Published In Biophysical Reviews
2018	Quantification of ligand density and stoichiometry on the surface of liposomes using single-molecule fluorescence imaging Published In Journal of Controlled Release
2017	Single-molecule visualization of Saccharomyces cerevisiae leading-strand synthesis reveals dynamic interaction between MTC and the replisome Published In Proceedings of the National Academy of Sciences of USA
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2017	Watching cellular machinery in action, one molecule at a time Published In The Journal of Cell Biology
2015	Single-molecule imaging at high fluorophore concentrations by local activation of dye Published In Biophysical Journal

Investigator On

Year	Title
2022 - 2026	Rapid evolution of a genome editing tool Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Investigator Grants

Other Research Activities

PERL Fellowship UOW PERL Fellow 2021 - 2022

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	Functional Implications of RarA at the Escherichia Coli Replication Fork
Doctor of Philosophy	Single-molecule studies of the bacterial replisome	Mueller, Stefan

Visualisations

Publication History

Investigator Network

Collaborator Map

Research Areas

0601 - BIOCHEMISTRY AND CELL BIOLOGY