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Mass Spectrometric Investigations of Conformation and Dynamics of Biological Complexes

Grant


Scheme


  • Linkage International

Abstract


  • The project will utilise ion mobility mass spectrometry (IMMS) to investigate the shape and size of complexes of the bacterial replisome. The replisome is a giant complex of proteins that efficiently copies the chromosome. Although the structures of many of the replisome proteins are known, the timing and dynamics of the assembly of the protein subunits are not understood. In this project IMMS will be used to investigate changes in shape and structure of subassemblies of the bacterial replisome to gain insight into the mechanism of this molecular machine. Ultimately this may lead to identification of new targets for antibiotics.

Date/time Interval


  • 2008

Sponsor Award Id


  • LX0882706

Local Award Id


  • 9828

Scheme


  • Linkage International

Abstract


  • The project will utilise ion mobility mass spectrometry (IMMS) to investigate the shape and size of complexes of the bacterial replisome. The replisome is a giant complex of proteins that efficiently copies the chromosome. Although the structures of many of the replisome proteins are known, the timing and dynamics of the assembly of the protein subunits are not understood. In this project IMMS will be used to investigate changes in shape and structure of subassemblies of the bacterial replisome to gain insight into the mechanism of this molecular machine. Ultimately this may lead to identification of new targets for antibiotics.

Date/time Interval


  • 2008

Sponsor Award Id


  • LX0882706

Local Award Id


  • 9828