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Mapping protein contacts and conformational changes in macromolecular assemblies

Grant


Scheme


  • Discovery Projects

Abstract


  • Although structures of proteins are being determined at an ever increasing rate, understanding how they interact and undergo changes in structure as they function in large dynamic complexes remains a serious challenge. We will exploit a series of new tools in protein chemistry including non-natural amino acid incorporation in cell-free expression systems and new chemical and spectroscopic methods to provide accurate distance measurements in protein complexes and complementary shape information. Using new computational tools, data from diverse experiments will be integrated to provide dynamic structural information on protein complexes that have resisted crystallization or are too big to study by protein NMR.

Date/time Interval


  • 2009

Sponsor Award Id


  • DP0984797

Local Award Id


  • 10346

Scheme


  • Discovery Projects

Abstract


  • Although structures of proteins are being determined at an ever increasing rate, understanding how they interact and undergo changes in structure as they function in large dynamic complexes remains a serious challenge. We will exploit a series of new tools in protein chemistry including non-natural amino acid incorporation in cell-free expression systems and new chemical and spectroscopic methods to provide accurate distance measurements in protein complexes and complementary shape information. Using new computational tools, data from diverse experiments will be integrated to provide dynamic structural information on protein complexes that have resisted crystallization or are too big to study by protein NMR.

Date/time Interval


  • 2009

Sponsor Award Id


  • DP0984797

Local Award Id


  • 10346