Collaborating Organisation
- Australian National University Research Organization
- The University Of Queensland
Mapping protein contacts and conformational changes in macromolecular assemblies
GrantOverview
Scheme
- Discovery Projects
Abstract
- Although structures of proteins are being determined at an ever increasing rate, understanding how they interact and undergo changes in structure as they function in large dynamic complexes remains a serious challenge. We will exploit a series of new tools in protein chemistry including non-natural amino acid incorporation in cell-free expression systems and new chemical and spectroscopic methods to provide accurate distance measurements in protein complexes and complementary shape information. Using new computational tools, data from diverse experiments will be integrated to provide dynamic structural information on protein complexes that have resisted crystallization or are too big to study by protein NMR.
Date/time Interval
- 2009
Awarded By
Contributor
- Dixon, Nicholas E. - Chief Investigator
- Tehei, Moeava - Chief Investigator
Sponsor Award Id
- DP0984797
Local Award Id
- 10346
Affiliation
Contributor
- Dixon, Nicholas E. - Chief Investigator
- Tehei, Moeava - Chief Investigator
Other
Assigned By
- Australian Research Council (ARC) Funding Organization
Overview
Collaborating Organisation
- Australian National University Research Organization
- The University Of Queensland
Scheme
- Discovery Projects
Abstract
- Although structures of proteins are being determined at an ever increasing rate, understanding how they interact and undergo changes in structure as they function in large dynamic complexes remains a serious challenge. We will exploit a series of new tools in protein chemistry including non-natural amino acid incorporation in cell-free expression systems and new chemical and spectroscopic methods to provide accurate distance measurements in protein complexes and complementary shape information. Using new computational tools, data from diverse experiments will be integrated to provide dynamic structural information on protein complexes that have resisted crystallization or are too big to study by protein NMR.
Date/time Interval
- 2009
Awarded By
Contributor
- Dixon, Nicholas E. - Chief Investigator
- Tehei, Moeava - Chief Investigator
Sponsor Award Id
- DP0984797
Local Award Id
- 10346
Affiliation
Contributor
- Dixon, Nicholas E. - Chief Investigator
- Tehei, Moeava - Chief Investigator
Other
Assigned By
- Australian Research Council (ARC) Funding Organization