Robinson, Andrew

Research Areas

Research Overview

Bacterial mutagenesis: single-molecule imaging and experimental evolution. Investigating the roles of error-prone DNA replication and repair processes in antibiotic resistance.

Available as Research Supervisor

Selected Publications

Journal Article

Year	Title
2021	Single-molecule fluorescence microscopy reveals modulation of DNA polymerase IV-binding lifetimes by UmuD (K97A) and UmuD' Published In Current Genetics
2021	Single-molecule localisation microscopy: accounting for chance co-localisation between foci in bacterial cells Published In European Biophysics Journal
2020	RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli Published In Nucleic Acids Research
2020	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2020	Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VICE391 Published In DNA Repair
2020	Antibiotic-Induced Mutagenesis: Under the Microscope Published In Frontiers in Microbiology
2020	Frequent template switching in postreplication gaps: suppression of deleterious consequences by the Escherichia coli Uup and RadD proteins Published In Nucleic Acids Research
2020	Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD Published In Nucleic Acids Research
2020	Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair Published In Nucleic Acids Research
2019	RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli Published In Nucleic Acids Research
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2019	Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VICE391 Published In DNA Repair
2018	DNA polymerase IV primarily operates outside of DNA replication forks in Escherichia coli Published In PLoS Genetics
2018	Specialised DNA polymerases in Escherichia coli: roles within multiple pathways Published In Current Genetics: lower eukaryotes and organelles
2018	Steric exclusion and protein conformation determine the localization of plasma membrane transporters Published In Nature Communications
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2016	A tool for alignment and averaging of sparse fluorescence signals in rod-shaped bacteria Published In Biophysical Journal
2016	Mutations for worse or better: low-fidelity DNA Synthesis by SOS DNA polymerase v is a tightly regulated double-edged sword Published In Biochemistry
2016	On the spatial organization of mRNA, plasmids, and ribosomes in a bacterial host overexpressing membrane proteins Published In PLoS Genetics
2016	Single-molecule specific mislocalization of red fluorescent proteins in live Escherichia coli Published In Biophysical Journal
2015	ISBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets Published In Molecular Biosystems
2015	Regulation of mutagenic DNA polymerase V activation in space and time Published In PLoS Genetics
2015	Single-molecule imaging at high fluorophore concentrations by local activation of dye Published In Biophysical Journal
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published In The EMBO Journal
2013	Bacterial replication, transcription and translation: mechanistic insights from single-molecule biochemical studies Published In Nature Reviews Microbiology
2013	Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain Published In Nucleic Acids Research
2012	Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target Published In Current Drug Targets
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published In Microbiology and Molecular Biology Reviews
2009	Identification of novel drug targets using model organisms Published In Microbiology Australia
2009	Lateral FtsZ association and the assembly of the cytokinetic Z ring in bacteria Published In Molecular Microbiology
2008	Structural genomics of the bacterial mobile metagenome: an overview. Published In Methods in molecular biology (Clifton, N.J.)
2007	A putative house-cleaning enzyme encoded within an integron array Published In Molecular Microbiology

Chapter

Year	Title
2008	Structural genomics of the bacterial mobile metagenome: an overview, in "Structural Proteomics: High Throughput Methods" Published In Methods in Molecular Biology Publisher NJ

Investigator On

Year	Title
2020 - 2021	Antibiotic resistance: combining microscopy, microfluidics and genomics to target bacterial DNA repair pathways Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2020 - 2021	Creation and Repair of Postreplicative DNA Gaps Awarded by: National Institute of Health (NIH) Funding Scheme: Grant
2020	Equipment Funding Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2018 - 2020	Molecular Interactions with an Antibiotic Target in DNA Replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2010	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Travel Grant
2010	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Travel Grant
2009	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Grant

Available as Research Supervisor

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	The role of translesion DNA synthesis in the development of antibiotic resistance	Cherry, Megan
Doctor of Philosophy	Visualising Single-Strand Gap Repair in Live Escherichia Coli Cells	Armstrong, Thomas
Doctor of Philosophy	Antibiotic-induced evolution: combining microscopy, microfluidics and genomics to study resistance in individual bacterial cells	Ripandelli, Remy
Doctor of Philosophy	Roles of Bacterial DNA Repair Pathways in Antibiotic Resistance	Wright, Elizabeth
Doctor of Philosophy	Single-molecule Imaging Analysis of Postreplicative Gap Formation and Repair in Escherichia coli	Belhaffef, Ouraida Amira

Organizer Of Event

Bugs by the Beach 2017 - 2018

Scopus Id

35619059900

Research Areas

Research Overview

Bacterial mutagenesis: single-molecule imaging and experimental evolution. Investigating the roles of error-prone DNA replication and repair processes in antibiotic resistance.

Selected Publications

Journal Article

Year	Title
2021	Single-molecule fluorescence microscopy reveals modulation of DNA polymerase IV-binding lifetimes by UmuD (K97A) and UmuD' Published In Current Genetics
2021	Single-molecule localisation microscopy: accounting for chance co-localisation between foci in bacterial cells Published In European Biophysics Journal
2020	RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli Published In Nucleic Acids Research
2020	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2020	Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VICE391 Published In DNA Repair
2020	Antibiotic-Induced Mutagenesis: Under the Microscope Published In Frontiers in Microbiology
2020	Frequent template switching in postreplication gaps: suppression of deleterious consequences by the Escherichia coli Uup and RadD proteins Published In Nucleic Acids Research
2020	Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD Published In Nucleic Acids Research
2020	Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair Published In Nucleic Acids Research
2019	RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli Published In Nucleic Acids Research
2019	Recycling of single-stranded DNA-binding protein by the bacterial replisome Published In Nucleic Acids Research
2019	Role of RNase H enzymes in maintaining genome stability in Escherichia coli expressing a steric-gate mutant of pol VICE391 Published In DNA Repair
2018	DNA polymerase IV primarily operates outside of DNA replication forks in Escherichia coli Published In PLoS Genetics
2018	Specialised DNA polymerases in Escherichia coli: roles within multiple pathways Published In Current Genetics: lower eukaryotes and organelles
2018	Steric exclusion and protein conformation determine the localization of plasma membrane transporters Published In Nature Communications
2017	Single-molecule visualization of fast polymerase turnover in the bacterial replisome Published In eLife
2016	A tool for alignment and averaging of sparse fluorescence signals in rod-shaped bacteria Published In Biophysical Journal
2016	Mutations for worse or better: low-fidelity DNA Synthesis by SOS DNA polymerase v is a tightly regulated double-edged sword Published In Biochemistry
2016	On the spatial organization of mRNA, plasmids, and ribosomes in a bacterial host overexpressing membrane proteins Published In PLoS Genetics
2016	Single-molecule specific mislocalization of red fluorescent proteins in live Escherichia coli Published In Biophysical Journal
2015	ISBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets Published In Molecular Biosystems
2015	Regulation of mutagenic DNA polymerase V activation in space and time Published In PLoS Genetics
2015	Single-molecule imaging at high fluorophore concentrations by local activation of dye Published In Biophysical Journal
2013	A direct proofreader-clamp interaction stabilizes the Pol III replicase in the polymerization mode Published In The EMBO Journal
2013	Bacterial replication, transcription and translation: mechanistic insights from single-molecule biochemical studies Published In Nature Reviews Microbiology
2013	Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, ε, θ and β reveals a highly flexible arrangement of the proofreading domain Published In Nucleic Acids Research
2012	Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target Published In Current Drug Targets
2010	Essential Biological Processes of an Emerging Pathogen: DNA Replication, Transcription, and Cell Division in Acinetobacter spp. Published In Microbiology and Molecular Biology Reviews
2009	Identification of novel drug targets using model organisms Published In Microbiology Australia
2009	Lateral FtsZ association and the assembly of the cytokinetic Z ring in bacteria Published In Molecular Microbiology
2008	Structural genomics of the bacterial mobile metagenome: an overview. Published In Methods in molecular biology (Clifton, N.J.)
2007	A putative house-cleaning enzyme encoded within an integron array Published In Molecular Microbiology

Chapter

Year	Title
2008	Structural genomics of the bacterial mobile metagenome: an overview, in "Structural Proteomics: High Throughput Methods" Published In Methods in Molecular Biology Publisher NJ

Investigator On

Year	Title
2020 - 2021	Antibiotic resistance: combining microscopy, microfluidics and genomics to target bacterial DNA repair pathways Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Project Grant
2020 - 2021	Creation and Repair of Postreplicative DNA Gaps Awarded by: National Institute of Health (NIH) Funding Scheme: Grant
2020	Equipment Funding Awarded by: National Health and Medical Research Council (NHMRC) Funding Scheme: Equipment Grant
2018 - 2020	Molecular Interactions with an Antibiotic Target in DNA Replication Awarded by: Australian Research Council (ARC) Funding Scheme: Discovery Projects
2010	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Travel Grant
2010	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Travel Grant
2009	DNA replication in pathogenic and model organisms: protein structure determination and ligand binding analyses Awarded by: Australian Synchrotron Research Program Funding Scheme: Grant

Advisees

Graduate Advising Relationship

Degree	Research Title	Advisee
Doctor of Philosophy	The role of translesion DNA synthesis in the development of antibiotic resistance	Cherry, Megan
Doctor of Philosophy	Visualising Single-Strand Gap Repair in Live Escherichia Coli Cells	Armstrong, Thomas
Doctor of Philosophy	Antibiotic-induced evolution: combining microscopy, microfluidics and genomics to study resistance in individual bacterial cells	Ripandelli, Remy
Doctor of Philosophy	Roles of Bacterial DNA Repair Pathways in Antibiotic Resistance	Wright, Elizabeth
Doctor of Philosophy	Single-molecule Imaging Analysis of Postreplicative Gap Formation and Repair in Escherichia coli	Belhaffef, Ouraida Amira

Organizer Of Event

Bugs by the Beach 2017 - 2018

Scopus Id

35619059900

Robinson, Andrew Dr

Research Fellow

Overview

Research Areas

Research Overview

Available as Research Supervisor

Publications

Selected Publications

Journal Article

Chapter

Funding

Investigator On

Supervision

Available as Research Supervisor

Advisees

Graduate Advising Relationship

Service

Organizer Of Event

Identity

Scopus Id

Overview

Research Areas

Research Overview

Publications

Selected Publications

Journal Article

Chapter

Funding

Investigator On

Supervision

Advisees

Graduate Advising Relationship

Service

Organizer Of Event

Identity

Scopus Id

Visualisations

Research Areas