Silla, Aimee J. Dr.

Postdoctoral Research Fellow

  • Faculty of Science, Medicine and Health
  • Centre for Sustainable Ecosystem Solutions
  • School of Biological Sciences

Research Overview


  • I am a Reproductive Biologist primarily focussed on developing Assisted Reproductive Technologies (ART) for the conservation of anurans. ART encompass a range of techniques that manipulate gametes for the purpose of controlling reproduction and enhancing fertility. My research includes manipulating hormone profiles to induce gamete-release, short-term gamete-storage, sperm cryopreservation, and artificial reproduction. I am also interested in comparative studies investigating response patterns among species, in order to expedite the application of ART to novel species.

     

    My research also employs evolutionary theory to predict ART outcomes. In particular, I am interested in investigating phenotypic indicators of male fertility, gamete adaptation to localised fertilisation environments, and how parental genetic incompatibilities effect fertilisation.

Selected Publications


Impact Story


  • <p>Environmental change is responsible for unprecedented rates of species extinction for all vertebrate classes, and amphibians have been most severely affected. Current estimates indicate that over 34 per cent of amphibian species globally are now threatened with extinction. The emerging methods being developed here at UOW integrate sophisticated Assisted Reproductive Technologies (ART) with traditional captive breeding methods to enhance the propagation and genetic management of threatened frog species.</p><p>This project began when UOW biologists Dr Phillip Byrne and Dr Aimee Silla were approached by the NSW Department of Environment and Heritage to develop ART for one of Australia’s most critically engendered vertebrates, the Southern Corroboree frog (<em>Pseudophryne corroboree</em>). Our research team has since developed ART for a number of model frog species as well as other species of high conservation value, including the critically endangered Booroolong frog (<em>Litoria booroolongensis</em>) and the Northern Corroboree frog (<em>Pseudophryne pengilleyi</em>).</p><p>Taronga Conservation Society Australia have adopted ART protocols developed by the researchers to hormonally induce spawning to enhance the captive breeding and release program for the Northern Corroboree frog. Overall, in excess of 800 viable eggs have been generated using ART over the past 3-years, and these eggs have been reintroduced into their natural environment by the NSW Office of Environment and Heritage, contributing significantly to the on-ground conservation efforts for this species. Our research team has also recently completed experiments at Taronga Zoo to develop protocols for the topical application of hormones to induce breeding so that ART technologies can be more widely adopted by global amphibian captive breeding facilities, without the need for specialist training in administering animal injections.</p><p>Our partner organisations have pledged additional funding over the next three years to further support these important avenues of research for improving endangered species captive breeding and reintroduction programs and ultimately, species recovery in the wild.</p>

Advisees


  • Graduate Advising Relationship

    Degree Research Title Advisee
    Doctor of Philosophy The evolutionary causes of sequential polyandry in terrestrial toadlets from the genus Pseudophryne O'Brien Jones, Daniel
    Doctor of Philosophy The influence of dietary carotenoids on the growth, development, colouration+ performance of the actually endangered Southern Corroboree frogs McInerney, Emma
    Doctor of Philosophy Understanding patterns of intersexual selection and individual behavioural variation to assist with the captive breeding and reintroduction of the critically endangered corroboree frog Kelleher, Shannon

Research Overview


  • I am a Reproductive Biologist primarily focussed on developing Assisted Reproductive Technologies (ART) for the conservation of anurans. ART encompass a range of techniques that manipulate gametes for the purpose of controlling reproduction and enhancing fertility. My research includes manipulating hormone profiles to induce gamete-release, short-term gamete-storage, sperm cryopreservation, and artificial reproduction. I am also interested in comparative studies investigating response patterns among species, in order to expedite the application of ART to novel species.

     

    My research also employs evolutionary theory to predict ART outcomes. In particular, I am interested in investigating phenotypic indicators of male fertility, gamete adaptation to localised fertilisation environments, and how parental genetic incompatibilities effect fertilisation.

Selected Publications


Impact Story


  • <p>Environmental change is responsible for unprecedented rates of species extinction for all vertebrate classes, and amphibians have been most severely affected. Current estimates indicate that over 34 per cent of amphibian species globally are now threatened with extinction. The emerging methods being developed here at UOW integrate sophisticated Assisted Reproductive Technologies (ART) with traditional captive breeding methods to enhance the propagation and genetic management of threatened frog species.</p><p>This project began when UOW biologists Dr Phillip Byrne and Dr Aimee Silla were approached by the NSW Department of Environment and Heritage to develop ART for one of Australia’s most critically engendered vertebrates, the Southern Corroboree frog (<em>Pseudophryne corroboree</em>). Our research team has since developed ART for a number of model frog species as well as other species of high conservation value, including the critically endangered Booroolong frog (<em>Litoria booroolongensis</em>) and the Northern Corroboree frog (<em>Pseudophryne pengilleyi</em>).</p><p>Taronga Conservation Society Australia have adopted ART protocols developed by the researchers to hormonally induce spawning to enhance the captive breeding and release program for the Northern Corroboree frog. Overall, in excess of 800 viable eggs have been generated using ART over the past 3-years, and these eggs have been reintroduced into their natural environment by the NSW Office of Environment and Heritage, contributing significantly to the on-ground conservation efforts for this species. Our research team has also recently completed experiments at Taronga Zoo to develop protocols for the topical application of hormones to induce breeding so that ART technologies can be more widely adopted by global amphibian captive breeding facilities, without the need for specialist training in administering animal injections.</p><p>Our partner organisations have pledged additional funding over the next three years to further support these important avenues of research for improving endangered species captive breeding and reintroduction programs and ultimately, species recovery in the wild.</p>

Advisees


  • Graduate Advising Relationship

    Degree Research Title Advisee
    Doctor of Philosophy The evolutionary causes of sequential polyandry in terrestrial toadlets from the genus Pseudophryne O'Brien Jones, Daniel
    Doctor of Philosophy The influence of dietary carotenoids on the growth, development, colouration+ performance of the actually endangered Southern Corroboree frogs McInerney, Emma
    Doctor of Philosophy Understanding patterns of intersexual selection and individual behavioural variation to assist with the captive breeding and reintroduction of the critically endangered corroboree frog Kelleher, Shannon
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